Strengthening antioxidant defense & cardio protection by Piper betle. - GreenMedInfo Summary
Strengthening antioxidant defense&cardio protection by: An in-vitro study.
Heliyon. 2020 Jan ;6(1):e03041. Epub 2019 Dec 26. PMID: 31909246
Introduction: The purpose of this research work was to evaluateethyl acetate extract (PBEA) for its free radical scavenging, antioxidant, anti-apoptotic activities and its role in protecting against oxidative cardiac cell injury.
Methods: The Free radical scavenging activity and antioxidant potential of PBEA were evaluated using various non-cellular methods (1,1-Diphenyl-2-picrylhydrazyl,β-carotene bleaching, superoxide anion, hydroxyl radical, hydrogen peroxideReducing power, Total phenolics and Total flavonoids). PBEA was standardized with Eugenol by GC-FID analysis. Furthermore, PBEA was also assessed for its cytotoprotective effect against 100μM HOin H9c2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Intracellular reactive oxygen species scavenging and anti-apoptoic activity of PBEA was assessed by using 2', 7'-Dichlorofluorescein diacetate and Annexin- Propidium Iodide, respectively.
Results: PBEA exhibited radical scavenging and antioxidant defense response at different magnitudes of potency. Eugenol, a cardiac protective bioactive molecule in PBEA was found to be 43.43± 1.46 mg/g of PBEA extract. Further, pre-incubation of H9c2 cells with 10 μg/ml PBEA for 24 h exhibited remarkable cytoprotective effect against HOinduced oxidative stress. PBEA at 10μg/ml dose with 24 h contact with H9c2 cells significantly enhanced the activity of cellular defense system and significantly decreased intracellular ROS (P<0.001) and apoptosis (P<0.01) thereby protecting against the cytotoxic effects of HO.
Conclusion: These outcomes indicated that PBEA could shield against oxidative and apoptotic cardiac cell injury in invitro studies. Thus, PBEA might be a desirable antioxidant of natural origin that has future clinical implications in both health care and food industry.