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Abstract Title:

Determination of melatonin and its isomer in foods by liquid chromatography tandem mass spectrometry.

Abstract Source:

Food Chem. 2014 Jun 15 ;153:151-6. Epub 2013 Dec 12. PMID: 24491714

Abstract Author(s):

Tolgahan Kocadağlı, Cemile Yılmaz, Vural Gökmen

Article Affiliation:

Tolgahan Kocadağlı

Abstract:

This study aimed to develop a reliable analytical method for the determination of melatonin and its isomers in various food products. The method entails ethanol extraction of solid samples (or dilution of liquid samples) prior to liquid chromatography coupled to triple quadruple mass spectrometry (LC-MS/MS) analysis of target analytes. The method was in-house validated and successfully applied to various food matrices. Recovery of melatonin from different matrices were found to be 86.0± 3.6%, 76.9 ± 5.4%, 98.6 ± 6.4%, and 67.0 ± 4.5% for beer, walnut, tomato and sour cherry samples, respectively. No melatonin could be detected in black and green tea, sour cherry, sour cherry concentrate, kefir (a fermented milk drink) and red wine while the highest amount of melatonin (341.7± 29.3 pg/g) was detected in crumb. The highest amounts of melatonin isomer were detected in yeast-fermented foods such as 170.7 ± 29.9 ng/ml in red wine, 14.3 ± 0.48 ng/ml in beer, and 15.7 ± 1.4 ng/g in bread crumb.

Study Type : In Vitro Study

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