Article Publish Status: FREE
Abstract Title:

The Analysis of the Anti-Tumor Mechanism of Ursolic Acid Using Connectively Map Approach in Breast Cancer Cells Line MCF-7.

Abstract Source:

Cancer Manag Res. 2020 ;12:3469-3476. Epub 2020 May 15. PMID: 32523377

Abstract Author(s):

Weiqiang Guo, Bin Xu, Xiaoxiao Wang, Bo Zheng, Jiahui Du, Songbai Liu

Article Affiliation:

Weiqiang Guo


Background: Ursolic acid (UA), a primary bioactive triterpenoid, was reported as an anti-cancer agent. However, the current knowledge of UA and its potential anti-cancer mechanisms and targets in breast cancer cells are limited. In this study, we aimed to illustrate the potential mechanisms and targets of UA in breast cancer cells MCF-7.

Methods: The effect of UA on cell growth was determined in MCF-7 cells by MTT assay. The anti-tumor mechanism of UA was evaluated by microarray, CAMP, and Western blot. Moreover, the molecular docking between UA and potential receptors were predicted by iGEMDOCK software.

Results: The result of MTT assay demonstrated that UA could inhibit MCF-7 cell growth with ICvalues of 20μM. Microarray and CMAP analysis, validated by Western blot, indicated that UA significantly modulated IKK/NF-κB, RAF/ERK pathways, and down-regulated the phosphorylation level of PLK1 in MCF-7 cells.

Conclusion: Our data indicated that the anti-tumor effects of UA are due to the inhibited RAF/ERK pathway and IKK/NF-κB pathway. It could also be explained by the reduced phosphorylation of PLK1 in MCF-7 cells. This study provides a new insight for deep understanding of the new anti-cancer mechanisms of UA in MCF-7 breast cancer cells.

Study Type : In Vitro Study

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