Evaluating the protective efficacy of Silybum marianum against deltamethrin induced hepatotoxicity in piscine model.
Environ Toxicol Pharmacol. 2019 Feb ;66:62-68. Epub 2018 Dec 21. PMID: 30605873
With the known facts of deltamethrin toxicity in aquaculture, efforts have been made to ameliorate its toxicity with supplementation of Silybum marianum plant extract in Cyprinus carpio. For validating its efficacy, standard techniques of histopathology, anti-oxidant profile and RAPD-PCR were used. By performing acute toxicity bioassay, 96 h LCof the pesticide to C. carpio was determined and was found to be 2μg/L. Histopathological alterations were comprised of nuclear alterations, vacuolisation and oedema in the hepatocytes. Chronic exposure to the toxicant induced significant changes in antioxidant defense system (CAT, SOD, GSH and GST levels), lipid peroxidation being prominent. Diet supplementationwith silymarin appeared to modulate the oxidative stress, histopathological alterations and genotoxic damage caused by the pesticide in the fish. RAPD-PCR studies revealed deltamethrin induced toxicity and its effective amelioration in form of restoration of bands which were lost in toxicant exposed DNA profile.