Article Publish Status: FREE
Abstract Title:

In Vitro Synergistic Inhibition of HT-29 Proliferation and 2H-11 and HUVEC Tubulogenesis by Bacopaside I and II Is Associated with CaFlux and Loss of Plasma Membrane Integrity.

Abstract Source:

Pharmaceuticals (Basel). 2021 May 6 ;14(5). Epub 2021 May 6. PMID: 34066415

Abstract Author(s):

Yoko Tomita, Eric Smith, Helen M Palethorpe, Maryam Nakhjavani, Kenny K L Yeo, Amanda R Townsend, Timothy J Price, Andrea J Yool, Jennifer E Hardingham

Article Affiliation:

Yoko Tomita


We previously showed how triterpene saponin bacopaside (bac) II, purified from the medicinal herb, induced cell death in colorectal cancer cell lines and reduced endothelial cell migration and tube formation, and further demonstrated a synergistic effect of a combination of bac I and bac II on the inhibition of breast cancer cell line growth. Here, we assessed the effects of bac I and II on the colorectal cancer HT-29 cell line, and mouse (2H-11) and human umbilical vein endothelial cell (HUVEC) lines, measuring outcomes including cell viability, proliferation, migration, tube formation, apoptosis, cytosolic Calevels and plasma membrane integrity. Combined bac I and II, each applied at concentrations below ICvalues, caused a synergistic reduction of the viability and proliferation of HT-29 and endothelial cells, and impaired the migration of HT-29 and tube formation of endothelial cells. A significant enhancement of apoptosis was induced only in HUVEC, although an increase in cytosolic Cawas detected in all three cell lines. Plasma membrane integrity was compromised in 2H-11 and HUVEC, as determined by an increase in propidium iodide staining, which was preceded by Caflux. These in vitro findings support further research into the mechanisms of action of the combined compounds for potential clinical use.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.