Abstract Title:

Patterning of antibodies using flexographic printing.

Abstract Source:

Langmuir. 2012 May 22. Epub 2012 May 22. PMID: 22616757

Abstract Author(s):

Christopher Owen Phillips, Sridhar Govindarajan, Simon M Hamblyn, Steven Conlan, David T Gethin, Tim Claypole

Abstract:

Antibodies were patterned onto flexible plastic films using the flexographic printing process. An ink formulation was developed using high molecular weight polyvinyl alcohol in carbonate-bicarbonate buffer. In order to aid both antibody adhesion and quality of definition in the printed features, a nitrocellulose coating was developed; this was capable of being discretely patterned thus increasing the signal to noise ratio of an antibody array. Printing antibody features such as dots, squares, text and fine lines were reproduced effectively. Furthermore, this process could be easily adapted for printing of other biological materials, including but not limited to, enzymes, DNA, proteins, aptamers and cells.

Study Type : Review

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