Histomorphometric studies of the effects ofon alcohol-induced gonado-toxicity in male rats.
Toxicol Rep. 2015 ;2:968-975. Epub 2015 Jun 18. PMID: 28962436
E N Akang
BACKGROUND: Available evidence suggests that 50% of couples with infertility are male related. Over 40% of these males consume alcohol which has been reported to be a reproductive toxicant causing depletions in the epithelium of seminiferous tubules hence reducing sperm counts and sperm morphology.
OBJECTIVE: To determine the effects of aqueous leaf extract ofon alcohol-induced cyto-architectural changes in the testis.
METHODS: Aqueous leaf extract of() was administered by gastric gavage at a dose of 250 mg/kg and 500 mg/kg body weight daily, while 2 g/kg body weight of ethanol at 30% v/v was administered daily to mature male Sprague-Dawley rats. The experiment was in 2 phases. Phase 1 had groups A-Fand lasted for 4 weeks while phase 2 had groups A-Fand lasted 8 weeks. Parameters tested include: testicular histology, relative volume density, sperm parameters, malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione.
RESULTS: In both phases, there were depletions in the seminiferous epithelium, decreased sperm quality and increased MDA and SOD in animals that received alcohol only compared to control. Likewise, a significant increase of seminiferous epithelium of animals that received respective doses of 250 mg/kg and 500 mg/kg ofonly compared to control. Animals that receivedand alcohol simultaneously had a significant increase in seminiferous epithelium and sperm quality with decreased MDA level.
CONCLUSION: attenuated the deleterious effects of alcohol to the cyto-architecture of the testis, protected the seminiferous epithelium, reduced oxidative stress and promoted spermatogenesis.