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Abstract Title:

Influence of Tanshinone IIA on the Apoptosis of Human Esophageal Ec-109 Cells.

Abstract Source:

Nat Prod Commun. 2016 Jan ;11(1):17-9. PMID: 26996008

Abstract Author(s):

Yan-Qin Zhu, Bai-Yan Wang, Fang Wu, Yong-Kang An, Xin-Qiang Zhou

Article Affiliation:

Yan-Qin Zhu

Abstract:

The induced-apoptosis effect and mechanism of human esophageal cancer Ec-109 cells via tanshinone IIA was investigated. The Ec-109 cells were cultured in vitro with different concentrations of tanshinone IIA (2µg/mL, 4 µg/mL, or 8 µg/mL) for 12, 24, 36, and 48 hours. MTT assay was used to evaluate the proliferative inhibition rate of tanshinone IIA on esophageal Ec-109 cells. After 24 hours of culturing in vitro, a control group was assigned. The apoptosis rate was detected by the AO/EB and annexin V-FITC/propidium iodide assay, and the protein levels of Caspase-4 and CHOP were determined by the Western blot technique. MTT data showed that tanshinone IIA could significantly inhibit the proliferation of Ec-109 cells with a dose- and time-dependent mode. Compared with the control group, tanshinoneIIA could apparently induce apoptosis of Ec-109 cells, and the level of Caspase-4 and CHOP (p<0.01) obviously increased. Tanshinone IIA can significantly induce the apoptosis of Ec-109 cells, which may take effect by the stress pathway of the endoplasmic reticulum.

Study Type : In Vitro Study

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Sayer Ji
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