Abstract Title:

Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells.

Abstract Source:

Food Nutr Res. 2016 ;60:30616. Epub 2016 Feb 17. PMID: 26899573

Abstract Author(s):

Xiao Han, Shan Deng, Ning Wang, Yafei Liu, Xingbin Yang

Article Affiliation:

Xiao Han

Abstract:

BACKGROUND: Tetrahydrocurcumin (THC), an active metabolite of curcumin, has been reported to have similar biological effects to curcumin, but the mechanism of the antitumor activity of THC is still unclear.

METHODS: The present study was to investigate the antitumor effects and mechanism of THC in human breast cancer MCF-7 cells using the methods of MTT assay, LDH assay, flow cytometry analysis, and western blot assay.

RESULTS: THC was found to have markedly cytotoxic effect and antiproliferative activity against MCF-7 cells in a dose-dependent manner with the IC50 for 24 h of 107.8μM. Flow cytometry analysis revealed that THC mediated the cell-cycle arrest at G0/G1 phase, and 32.8% of MCF-7 cells entered the early phase of apoptosis at 100 μM for 24 h. THC also dose-dependently led to apoptosis in MCF-7 cells via the mitochondrial pathway, as evidenced by the activation ofcaspase-3 and caspase-9, the elevation of intracellular ROS, a decrease in Bcl-2 and PARP expression, and an increase in Bax expression. Meanwhile, cytochrome C was released to cytosol and the loss of mitochondria membrane potential (Δψm) was observed after THC treatment.

CONCLUSION: THC is an excellent source of chemopreventive agents in the treatment of breast cancer and has excellent potential to be explored as antitumor precursor compound.

Study Type : In Vitro Study

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Sayer Ji
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