Abstract Title:

Antiviral activity of selected flavonoids against Chikungunya virus.

Abstract Source:

Antiviral Res. 2016 Jul 25 ;133:50-61. Epub 2016 Jul 25. PMID: 27460167

Abstract Author(s):

Rafidah Lani, Pouya Hassandarvish, Meng-Hooi Shu, Wai Hong Phoon, Justin Jang Hann Chu, Stephen Higgs, Dana Vanlandingham, Sazaly Abu Bakar, Keivan Zandi

Article Affiliation:

Rafidah Lani

Abstract:

This study focuses on the antiviral activity of selected flavonoids against the Chikungunya virus (CHIKV), a mosquito-transmitted virus that can cause incapacitating arthritis in infected individuals. Based on the results of screening on Vero cells, the tested compounds were evaluated further with various assays, including cytotoxicity assay, virus yield assay by quantitative reverse transcription polymerase chain reaction (qRT-PCR), virus RNA replication assay with a CHIKV replicon cell line, Western blotting, and quantitative immunofluorescence assay. Baicalein, fisetin, and quercetagetin displayed potent inhibition of CHIKV infection, with 50% inhibitory concentrations [IC50] of 1.891 μg/ml (6.997 μM), 8.444 μg/ml (29.5 μM), and 13.85 μg/ml (43.52 μM), respectively, and with minimal cytotoxicity. The time-of-addition studies and various antiviral assays demonstrated that baicalein and quercetagetin mainly inhibited CHIKV binding to the Vero cells and displayed potentactivity against extracellular CHIKV particles. The qRT-PCR, immunofluorescence assay, and Western blot analyses indicated that each of these flavonoids affects CHIKV RNA production and viral protein expression. These data provide the first evidence of the intracellular anti-CHIKV activity of baicalein, fisetin, and quercetagetin.

Study Type : In Vitro Study

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