L. Extract Induces Antibacterial Activity and Modulates Oxidative Stress, Inflammation, and Apoptosis in Endothelial Vascular Cells Exposed to Hyperosmotic Stress.
Oxid Med Cell Longev. 2018 ;2018:3060525. Epub 2018 Feb 14. PMID: 29636839
Background: The antimicrobial activity of theL. extract and the mechanisms involved in theeffects on endothelial vascular cells exposed to hyperosmotic stress were evaluated.
Methods: Antimicrobial activity was evaluated by disk diffusion method and minimum inhibitory concentration (MIC) determination, and oxidative stress, inflammation, and apoptosis, in pretreatment withL., caffeic acid, and cathechin, were quantified.
Results: The results have shown thatL. exhibited antibacterial effects only on pathogenic gram-positive cocci. The modulatory activity ofL. on endothelial cells exposed to hypertonic medium was different and depended on the concentration used. Low concentrations of tested compounds exerted antioxidant effect and diminished the activity of caspase-8 and also increased IB expression while in high doses,L. was prooxidant, induced apoptosis, and decreased IL-6 secretion.
Conclusions: These experimental findings suggest thatL. has antibacterial effects on gram-positive cocci and, administered in low dose, may be a new therapeutic approach for diseases associated with hypertonic conditions or oxidative stress and apoptosis.