Inhibitory Effect of Thymoquinone onATCC 19115 Biofilm Formation and Virulence Attributes Critical for Human Infection.
Front Cell Infect Microbiol. 2019 ;9:304. Epub 2019 Aug 27. PMID: 31508379
This study aimed to determine the antimicrobial activity of thymoquinone (TQ) against, and to examine its inhibitory effects on biofilm formation, motility, hemolysin production, and attachment-invasion of host cells. The minimum inhibitory concentrations (MICs) of TQ against eight differentstrains ranged from 6.25-12.50μg/mL. Crystal violet staining showed that TQ clearly reduced biofilm biomass at sub-MICs in a dose-dependent manner. Scanning electron microscopy suggested that TQ inhibited biofilm formation on glass slides and induced an apparent collapse of biofilm architecture. At sub-MICs, TQ effectively inhibited the motility ofATCC 19115, and significantly impacted adhesion to and invasion of human colon adenocarcinoma cells as well as the secretion of listeriolysin O. Supporting these findings, real-time quantitative polymerase chain reaction analysis revealed that TQ down-regulated the transcription of genes associated with motility, biofilm formation, hemolysin secretion, and attachment-invasion in host cells. Overall, these findings confirm that TQ has the potential to be used to combatinfection.