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Abstract Title:

Secondary Metabolites (Essential Oils): In Vitro and In Silico Studies on Human Hepatocellular Carcinoma Cell Lines.

Abstract Source:

Plants (Basel). 2021 Sep 9 ;10(9). Epub 2021 Sep 9. PMID: 34579396

Abstract Author(s):

Shaimaa Nazir, Ahmed A El-Sherif, Nour T Abdel-Ghani, Mahmoud A A Ibrahim, Mohamed-Elamir F Hegazy, Mohamed A M Atia

Article Affiliation:

Shaimaa Nazir

Abstract:

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and the greatest cause of cancer-related death in the world. Garden cress () seeds have been proven to possess extraordinary antioxidant, anti-inflammatory, hypothermic, and analgesic properties. In this study, in vitro cytotoxic efficiency evaluation offractions was performed against two hepatocellular carcinoma cell lines (HuH-7 and HEPG-2), and the expression of some apoptotic genes was explored. In addition, the chemical composition of a potent extract ofwas analyzed using gas chromatography coupled with mass spectrometry. Then, molecular docking analysis was implemented to identify the potential targets of thecomponents' most potent extract. Overall, the-hexane extract was the most potent against the two HCC cell lines. Moreover, these cytotoxicity levels were supported by the significant downregulation of EGFR and BCL2 gene expression levels and the upregulation of SMAD3, BAX, and P53 expression levels in both HuH-7 and HEPG2 cell lines. Regarding's chemical composition, GC-MS analysis of the-hexane extract led to the identification of thirty compounds, including, mainly, hydrocarbons and terpenoids, as well as other volatile compounds. Furthermore, the binding affinities and interactions of the-hexane fraction's major metabolites were predicted against EGFR and BCL2 molecular targets using the molecular docking technique. These findings reveal the potential use ofin the management of HCC.

Study Type : In Vitro Study

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