Bactericidal Effect of the Leaf Extract fromspp. (AAB Group, Silk Subgroup), cv."Manzano"Against Multidrug-Resistant.
J Med Food. 2019 Jul 10. Epub 2019 Jul 10. PMID: 31268391
Gloria María Molina-Salinas
Air-dried leaves of aspp. AAB, cv."Manzano"plant, known asin the Maya culture, were sequentially extracted with hexane, ethyl acetate, and methanol; the resulting extracts were investigated for their antimycobacterial activity against susceptible and drug-resistant strains of(MTB) using the Microplate Alamar Blue Assay. Both the n-hexane extract (HE) and ethyl acetate extract (EE) showed potent activity against both strains of MTB, with the EE exhibiting the strongest activity and a Minimum Inhibitory Concentration of 12.5 and 6.25 μg/mL against susceptible and drug-resistant strains, respectively. Both extracts also demonstrated a mycobactericidal effect and a very good selectivity index when tested for cytotoxic activity on Vero monkey kidney cells, using the Sulforhodamine B assay. Our results demonstrate the efficiencyand selectivity ofspp. AAB, cv."Manzano"against MTB strains and support its traditional use as remedy against tuberculosis in Maya traditional medicine.