APC 678 Reduces Shedding of the Pathogenin a Murine Model.
Front Microbiol. 2019 ;10:273. Epub 2019 Feb 20. PMID: 30842760
is a common cause of health-care acquired diarrhea, resulting in a spectrum of disease from mild diarrhea to life-threatening illness. Sixtystrains were screened for anti-activity using a co-culture method. Based on their ability to inhibit,APC 678 andDPC 6111 were selected for study in a murine model ofinfection.ATCC 33323, was included as a control. It was established that, relative to control mice not fed, feeding withAPC 678 resulted in a significant reduction by day 7 (8-fold,= 0.017) of viableVPI 10463 in the feces of mice. In contrast, neitherDPC 6111 norATCC 33323 significantly reduced fecalshedding. Sequencing of the cecal microbiota showed that in mice fedAPC 678 there was a significant increase in bacterial diversity across a number of indices when compared to the control or other-fed groups. There was no significant change in the relative abundance of Firmicutes or Bacteroidetes in the group fedAPC 678 relative to the control, while the groups fedDPC 6111 orATCC 33323 showed a significant decrease in the relative abundance of Firmicutes (= 0.002 and= 0.019, respectively) and a significant increase in Bacteroidetes (= 0.002 and= 0.023, respectively). These results highlight the potential ofAPC 678 as a live therapeutic agent to targetinfection.