Abstract Title:

Effect of thimerosal, a preservative in vaccines, on intracellular Ca2+ concentration of rat cerebellar neurons.

Abstract Source:

Toxicology. 2004 Jan 15 ;195(1):77-84. PMID: 14698570

Abstract Author(s):

Toshiko Ueha-Ishibashi, Yasuo Oyama, Hiromi Nakao, Chisato Umebayashi, Yasutaka Nishizaki, Tomoko Tatsuishi, Kyoko Iwase, Koji Murao, Hakaru Seo

Article Affiliation:

Toshiko Ueha-Ishibashi

Abstract:

The effect of thimerosal, an organomercurial preservative in vaccines, on cerebellar neurons dissociated from 2-week-old rats was compared with those of methylmercury using a flow cytometer with appropriate fluorescent dyes. Thimerosal and methylmercury at concentrations ranging from 0.3 to 10 microM increased the intracellular concentration of Ca2+ ([Ca2+]i) in a concentration-dependent manner. The potency of 10 microM thimerosal to increase the [Ca2+]i was less than that of 10 microM methylmercury. Their effects on the [Ca2+]i were greatly attenuated, but not completely suppressed, under external Ca(2+)-free condition, suggesting a possibility that both agents increase membrane Ca2+ permeability and release Ca2+ from intracellular calcium stores. The effect of 10 microM thimerosal was not affected by simultaneous application of 30 microM L-cysteine whereas that of 10 microM methylmercury was significantly suppressed. The potency of thimerosal was similar to that of methylmercury in the presence of L-cysteine. Both agents at 1 microM or more similarly decreased the cellular content of glutathione in a concentration-dependent manner, suggesting an increase in oxidative stress. Results indicate that thimerosal exerts some cytotoxic actions on cerebellar granule neurons dissociated from 2-week-old rats and its potency is almost similar to that of methylmercury.

Study Type : In Vitro Study
Additional Links
Problem Substances : Thimerosal : CK(618) : AC(0)
Adverse Pharmacological Actions : Neurotoxic : CK(1458) : AC(0)

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