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Abstract Title:

Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B.

Abstract Source:

Evid Based Complement Alternat Med. 2018 ;2018:1549805. Epub 2018 Aug 16. PMID: 30186351

Abstract Author(s):

Aminah Suhaila Haron, Sharifah Sakinah Syed Alwi, Latifah Saiful Yazan, Rohaina Abd Razak, Yong Sze Ong, Fatin Hannani Zakarial Ansar, Henna Roshini Alexander

Article Affiliation:

Aminah Suhaila Haron

Abstract:

Thymoquinone (TQ), a bioactive compound found in, cannot be orally consumed due to its lipophilicity. In order to overcome this low bioavailability, TQ is loaded into a colloidal drug carrier known as a nanostructured lipid carrier (NLC). This study aims to determine the antiproliferative effects of TQ and TQ-NLC on liver cancer cells integrated with the hepatitis B genome, Hep3B. The Hep3B was treated with TQ or TQ-NLC for 24, 48, and 72 hours via MTT assay. The results confirm that TQ or TQ-NLC inhibited the growth of Hep3B at IC50<16.7M for 72 hours. TQ was also found to induce cell cycle arrest at the G1 checkpoint while TQ-NLC induced non-phase-specific cell cycle arrest. Further analysis using Annexin V staining confirmed the apoptotic induction of TQ or TQ-NLC via activation of caspases-3/7. In ROS management, TQ acted as a prooxidant (increased the level of ROS), while TQ-NLC acted as an antioxidant (reduced the level of ROS). Molecular analysis demonstrated that the GSH system and the Nrf2/Keap1 signaling pathway in Hep3B influenced the differential responses of the cells towards TQ or TQ-NLC. Hence, this study demonstrated that TQ and TQ-NLC haveanticancer effects on the Hep3B.

Study Type : In Vitro Study

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