Benefits of Bifidobacterium Animalis Subsp Lactis Probiotic in Experimental Periodontitis.
J Periodontol. 2016 Sep 23:1-20. Epub 2016 Sep 23. PMID: 27660886
Luiz F F Oliveira
BACKGROUND: This study evaluated the effects of topical administration of probiotic bacteria (PROB) of the genus Bifidobacterium on experimental periodontitis (EP) in rats.
METHODS: 32 rats were divided into groups C (control; without EP), EP (EP only), C-HN019 (control+PROB) and EP-HN019 (EP+PROB). On day 0 of the experiment, animals of groups EP and EP-HN019 received cotton ligatures around mandibular first molars (MFM). In groups C-HN019 and EP-HN019, 1mL of suspensions containing Bifidobacterium animalis subsp lactis (B. lactis) HN019 was topically administered in the subgingival region of MFM on days 0, 3 and 7. In groups C and EP, topical administrations were performed using a sham suspension (without PROB). All animals were euthanized at day 14. Gingival tissue, hemi-mandibles and oral biofilm were collected. Data were statistically analyzed (P<0.05).
RESULTS: Group EP presented greater bone porosity, trabecular separation and connective tissue attachment loss as well as reduced bone volume than all the other groups (P<0.05). In group EP-HN019, there were greater proportions of Actinomyces and Streptococcus-like species and lower proportions of Veillonella parvula, Capnocytophaga sputigena, Eikenella corrodens, Prevotella intermedia-like species than group EP. Besides, group EP-HN019 presented greater expressions of Osteoprotegerin (OPG) and beta-defensins than group EP (P<0.05). Group EP presented greater levels of Interleukin (IL)-1β and Receptor Activator of Nuclear Factor-Kappa B ligand (RANKL) than group EP-HN019 (P<0.05).
CONCLUSION: Topical use of B. lactis HN019 promotes a protective effect against alveolar bone and connective tissue attachment losses attributable to EP in rats, modifying immunoinflammatory and microbiological parameters.