may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN-45.
Oncol Lett. 2017 Feb ;13(2):841-846. Epub 2016 Dec 20. PMID: 28356967
Gastric carcinoma (GC) is one of the most common malignant tumors and is mainly treated by invasive surgeries. The present study aimed to investigate the treatment potential ofon GC using the human GC cell line MKN-45. Cells were incubated withat a concentration of 0, 5 and 10 mg/ml for 24 h. The apoptosis of the cell line was examined with acridine orange/ethidium bromide staining and flow cytometry. The expression of B-cell lymphoma (Bcl)-2, Fas, caspase-3, matrix metalloproteinase (MMP)-2 and MMP-9 was analyzed using reverse transcription-polymerase chain reaction and western blotting. With increasing drug concentrations, the proportion of apoptotic and necrotic cells increased. For a certain concentration, the apoptotic ratio also increased with increasing response times. Compared with the control group, the,andexpression levels in the MKN-45 cell line decreased, while the expression levels ofandincreased (P<0.05), and the expression patterns were strengthened with increasing drug concentrations. The present study revealed thathad treatment potential on GC, and it may act on gastric cells through apoptotic induction and MMPs expression inhibition. Based on the present results,may be considered as an alternative approach for noninvasive therapy of GC. However, future studies should be performed to clarify this further.