A Trypsin Inhibitor fromFlowers Modulates the Immune Response In Vitro of-Infected Human Cells.
Antibiotics (Basel). 2020 Aug 14 ;9(8). Epub 2020 Aug 14. PMID: 32823803
Isabella Coimbra Vila Nova
causes the lethal Chagas disease, which is endemic in Latin America. Flowers of(Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity totrypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by-infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LCof 43.5µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index>11.48 was determined. When-infected PBMCs were treated with MoFTI (43.5 or 87.0µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity toand the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease.