Ursolic Acid could be useful in the co-treatment of Tuberculosis infections. - GreenMedInfo Summary
Ursolic Acid activates intracellular killing Effect of Macrophage in Mycobaterium tuberculosis infection.
J Microbiol Biotechnol. 2014 Nov 19. Epub 2014 Nov 19. PMID: 25406534
Tuberculosis is one of the most threatening infectious diseases to public health all over the world, for which Mycobacterium tuberculosis is the etiological agent of pathogenesis. Ursolic acid (UA) has immunomodulatory function and exhibits antimycobacterial activity. However, the intracellular killing effect of UA has yet to be elucidated. The aim of this study was to evaluate the intracellular killing effect of UA during mycobacterial infection. The intracellular killing activity of UA was evaluated in the macrophage cell line THP-1 by the MGIT 960 system as well as by CFU count. The production of Reactive Oxygen Species (ROS) and the level of nitric oxide (NO) were measured using DCF-DA and Griess reagent, respectively. Phagocytosis assay was observed by a fluorescence-based staining method, and the colony forming units were enumerated on 7H11 agar media following infection. In addition, MRP8 mRNA expression was measured by qRTPCR. UA significantly decreased the number of intracellular Mycobacterium through generation of ROS and NO. In addition, it profoundly activated the phagocytosis process of THP-1 cells during Mtb-infection. Furthermore, our data demonstrated that UA activated the phagocytosis process in human monocyte cells through MRP8 induction. These data suggest that UA firmly contributes to the intracellular killing effect of macrophages during mycobacterial infection.