In vitro and ex vivo antiparasitic effect of Rheum ribes L. extract against the hydatid cyst protoscoleces.
Infect Disord Drug Targets. 2020 Nov 15. Epub 2020 Nov 15. PMID: 33200714
BACKGROUND: Cystic echinococcosis is a zoonotic infection in humans and herbivorous animals with worldwide distribution which caused by larva stage of Echinococcus granulosus. Rhubarb (Rheum ribes L.) as an herbal medicines has various therapeutics properties such as the antioxidant, anticancer, and antimicrobial ones. With respect to the potential of the biological activities of this plant in traditional and modern medicine, we aim to examine its protoscolicidal effects against E. granulosus protoscolecess in vitro and ex vivo.
METHODS: Collected protoscoleces from liver hydatid cysts of infected sheep were exposed to the different concentrations of the extract (225, 450, 900 mg/mL) for 5-60 min in vitro and ex vivo. Then using the eosin exclusion assay the viability of protoscoleces was studied.
RESULTS: R. ribes extract had a potent protoscolicidal activity in vitro so that at the 450 and 900 mg/ml killed 56.3 and 100% of protoscoleces after 10 min exposure. Ex vivo assay, the extract needed more time to kill the protoscoleces than the in vitro; so that at the concentration of 900 mg/mL, all protoscoleces were killed after 15 minutes.
CONCLUSION: The obtained results exhibited the potent protoscolicidal effects of R. ribes extract particularly at the concentration of 900 mg/ml which completely killed the parasite after<15 min exposure. However, more and supplementary studies are required to verify these findings through assessing in animal models and clinical subjects.