In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus plantarum pH3A, monolaurin, and grapefruit seed extract. - GreenMedInfo Summary

infection is the most common cause of gastritis and gastric ulcers. Considering the severe side effects of current antibiotic therapies, it is crucial to find an alternate treatment forinfection. In this study, we investigated the anti-effects of a newly isolated strain of(pH3A), monolaurin, grapefruit seed extract (GSE), and their synergiesand. Monolaurin and GSE suppressedgrowth and urease activity at a minimal inhibitory concentration (MIC) of 62.5 ppm. Live cells and cell-free culture supernatant (CFCS) ofpH3A with or without pH adjustment also significantly inhibitedgrowth. Although synergy was not observed between monolaurin and GSE, the addition of CFCS significantly enhanced their anti-activities. Moreover,pH3A significantly decreased the ability ofto adhere to AGS cells and interleukin (IL)-8 production in the-stimulated AGS cell line. The addition of GSE or monolaurin strengthened these effects. In thestudy,colonization of the mouse stomach and total serum IgG production were significantly reduced bypH3A treatment, but the addition of monolaurin or GSE did not contribute to these anti-activities. Therefore, thepH3A strain can potentially be applied as an alternative anti-therapy, but evidence of its synergy with monolaurin or GSEis still lacking.