Abstract Title:

[Effects of Wogonin on Apoptosis,Invasion,Migration and Wnt/β-Catenin Signaling Pathway of Gastric Cancer Cells SGC7901].

Abstract Source:

Zhong Yao Cai. 2016 Jun ;39(6):1372-6. PMID: 30156810

Abstract Author(s):

Xiao-hui Wang, Ying-fang Wei, Hong Cheng

Article Affiliation:

Xiao-hui Wang


Objective: To explore the effects of wogonin on the apoptosis,invasion,migration and Wnt /β-catenin signaling pathway of gastric cancer cells SGC7901.

Methods: Three common gastric cancer cell lines( SGC7901,BGC-823 and MKN-45) were conventionally cultured to logarithmic growth. The cell proliferation was detected by MTT assay after treatment with different concentrations of wogonin( 0,20,50,100 and 200μmol / L) for 24,48,72 and 96 h. The apoptosis rate, migration and invasion ability of SGC7901 cells were detected by Annexin V-FITC / PI annexin double staining flow cytometry, scratch test and transwell cell invasion assay, respectively. After treament with different concentrations of wogonin for48 h,the protein levels of β-catenin, C-myc and Cyclin D1 were detected by Western blotting.

Results: Wogonin within the range of 20 ~ 200 mol / L could inhibit the proliferation of SGC7901,BGC-823 and MKN-45 cells in a dose-and time-dependent manner. Compared with 0μmol / L wogonin group,After treament with different concentrations of wogonin for 24 h and 48 h,SGC7901 cells had elevated apoptosis rate and decreased migration distance and number of penetrating membrane cell in the rest concentrations( P<0. 05). The protein levels ofβ-catenin,C-myc and Cyclin D1 were lower in 20 ~ 200μmol /L wogonin groups than 0 μmol /L wogonin group except for the concentration of 20 μmol /L wogonin groups( P<0. 05),and the effects in a dose-dependent manner.

Conclusion: Wogonin has the ability to inhibit the proliferation of gastric cancer cells,and can induce apoptosis and inhibit cell migration and invasion,which may be related to the inhibition of Wnt /β-catenin signaling pathway activation.

Study Type : In Vitro Study

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