The hydroalcoholic extract ofdiminishes diazinon-induced hepatotoxicity by suppressing oxidative stress and apoptosis in rats.
Biotech Histochem. 2020 Jul 16:1-7. Epub 2020 Jul 16. PMID: 32672073
Saeed Yaghubi Beklar
Diazinon (DZN) is an organophosphate insecticide that affects the liver adversely. Ginger exhibits antioxidant properties. We investigated the hepatoprotective effects of an ethanolic extract of ginger root on DZN induced hepatotoxicity. We measured total phenolics and flavonoids in the hydroalcoholic extract. We used Wistar rats divided into four groups: control, 100 mg/kg/day ginger by gavage, 10 mg/kg/day DZN intraperitoneally, and ginger + DZN group treated with ginger 1 h before DZN. All treatments were for 30 consecutive days. One day after the last treatment, we evaluated oxidative stress parameters, serum biochemistry, histopathology and immunohistochemistry. The ginger extract contained 101.33 ± 2.73 mg total flavonoid and 237.9 ± 3 mg total phenolic content/g dry ginger plant roots. We found that DZN increased oxidative stress significantly. Histopathology of the liver tissue was consistent with increased AST, ALT, and ALP. Ginger extract treatment reduced oxidative stress and improved histopathology. DZN increased caspase-3 immunoreactivity andginger extract reduced it. Ginger extract exhibited hepatoprotective effects against DZN induced hepatic injury owing to its antioxidant and anti-apoptotic activity.