Zingiber officinale ethanolic extract attenuated reserpine-induced depression. - GreenMedInfo Summary
Ethanolic Extract Attenuated Reserpine-Induced Depression-Like Condition and Associated Hippocampal Aberrations in Experimental Wistar Rats.
J Exp Pharmacol. 2020 ;12:439-446. Epub 2020 Nov 2. PMID: 33173355
John Afees Olanrewaju
Background: Repeated and regimented treatment with reserpine causes depression-like condition characterized by persistent mood disorder, feelings of severe despondency and dejection, thus altering the hippocampal morphology. Our study compared a well-known antidepressant (fluoxetine), with the potential ofto ameliorate reserpine-induced depression and the associated hippocampal cornu ammonis 1 (CA1) neuronal cell damage.
Methods: Forty-eight male Wistar rats, weighing 130-160 g, were randomly assigned to 6 groups (n=8), housed in plastic cages under natural light and dark cycles at room temperature with access to feed and water. Group-A (control) received distilled water. Group-B and Group-C orally received 400 mg/kg ofand 10 mg/kg of fluoxetine, respectively, for 7 days, while Group-D intraperitoneally received 0.2 mg/kg of reserpine for 14 days. Group-E and Group-F intraperitoneally received 0.2 mg/kg of reserpine for 14 days followed by 400 mg/kg ofand 10 mg/kg of fluoxetine respectively for 7 days. All animals were sacrificed by cervical dislocation at the end of experiment, and the brains hippocampi were dissected, excised and processed for various analyses including histology [H&E], histochemistry of GFAP expression by astrocytes and specific gene expressions including p53 gene, glutathione reductase (GSR), glutathione peroxidase and catalase (CAT).
Results: Reserpine significantly depleted the expression of P53 and glutathione reductase (GSR) genes while significantly increasing the expression of glutathione peroxidase 1 (GPx-1) gene (P≤0.05). Also, a marked increase in the expression of catalase (CAT) gene was observed. Furthermore, histoarchitecture (photomicrographs) of hippocampus CA1 region showed disruption in the arrangement of pyramidal neurons and alterations in their morphologies when animals were treated with reserpine (Group D). There was also accompanying increased astrocyte densities within the CA1 region following reserpine treatment. These features indicated deleterious effects of reserpine. Bothand fluoxetine treatments ameliorated these effects.
Conclusion: These findings showed structural and molecular alterations associated with reserpine-induced depression. Also,was effective to provide ameliorative and protective effects against the neurotoxic effects of reserpine in the hippocampus, making it a potential candidate for treating depression and its associated neurodegenerative diseases.