Antioxidant https://greenmedinfo.com/category/pharmacological-actions/antioxidant en 1800 MHz increases oxidative stress and apoptosis in mouse-derived sperm cells, but N-acetyl cysteine and autophagy prevented EMF-associated cell death. https://greenmedinfo.com/article/1800-mhz-increases-oxidative-stress-and-apoptosis-mouse-derived-sperm-cells-n- PMID:  Toxicol Lett. 2014 Aug 4 ;228(3):216-24. Epub 2014 May 9. PMID: 24813634 Abstract Title:  The protective effect of autophagy on mouse spermatocyte derived cells exposure to 1800MHz radiofrequency electromagnetic radiation. Abstract:  The increasing exposure to radiofrequency (RF) radiation emitted from mobile phone use has raised public concern regarding the biological effects of RF exposure on the male reproductive system. Autophagy contributes to maintaining intracellular homeostasis under environmental stress. To clarify whether RF exposure could induce autophagy in the spermatocyte, mouse spermatocyte-derived cells (GC-2) were exposed to 1800MHz Global System for Mobile Communication (GSM) signals in GSM-Talk mode at specific absorption rate (SAR) values of 1w/kg, 2w/kg or 4w/kg for 24h, respectively. The results indicated that the expression of LC3-II increased in a dose- and time-dependent manner with RF exposure, and showed a significant change at the SAR value of 4w/kg. The autophagosome formation and the occurrence of autophagy were further confirmed by GFP-LC3 transient transfection assay and transmission electron microscopy (TEM) analysis. Furthermore, the conversion of LC3-I to LC3-II was enhanced by co-treatment with Chloroquine (CQ), indicating autophagic flux could be enhanced by RF exposure. Intracellular ROS levels significantly increased in a dose- and time-dependent manner after cells were exposed to RF. Pretreatment with anti-oxidative NAC obviously decreased the conversion of LC3-I to LC3-II and attenuated the degradation of p62 induced by RF exposure. Meanwhile, phosphorylated extracellular-signal-regulated kinase (ERK) significantly increased after RF exposure at the SAR value of 2w/kg and 4w/kg. Moreover, we observed that RF exposure did not increase the percentage of apoptotic cells, but inhibition of autophagy could increase the percentage of apoptotic cells. These findings suggested that autophagy flux could be enhanced by 1800MHz GSM exposure (4w/kg), which is mediated by ROS generation. Autophagy may play an important role in preventing cells from apoptotic cell death under RF exposure stress. <p><a href="https://greenmedinfo.com/article/1800-mhz-increases-oxidative-stress-and-apoptosis-mouse-derived-sperm-cells-n-" target="_blank">read more</a></p> https://greenmedinfo.com/article/1800-mhz-increases-oxidative-stress-and-apoptosis-mouse-derived-sperm-cells-n-#comments Infertility Infertility: Male NAC (N-acetyl-L-cysteine) Oxidative Stress Radiation Induced Illness Sperm Count: Low Sperm Quality: Low Antioxidant Apoptotic Cell Phone Exposure Electromagnetic Field Harms Electromagnetic Radiation Electromagnetic Radiation: 1.8 GHz Microwave Radiation Mobile Phone Radiation Oxidant Autophagy Autophagy Up-regulation Cell Phones electromagnetic fields electromagnetic frequencies electromagnetic radiation EMF infertility male infertility Male Infertility and Cellular Phones Radio Frequency Electromagnetic Radiation Exposure In Vitro Study Fri, 02 Aug 2019 20:17:48 +0000 greenmedinfo 192634 at https://greenmedinfo.com 2 Skin Aging Reversal Remedies Proven Clinically https://greenmedinfo.com/blog/2-skin-aging-reversal-remedies-proven-clinically <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2024<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter" dir="ltr"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/208797790_m_normal_none.jpg" style="width: 600px; height: 315px;" /></p> <p dir="ltr"><span style="font-size:18px;"><strong><em>Too many of us believe that our rate of aging is predetermined and inevitable. Yet, the truth is that our exact biological age is determined not by our genes, but more by what we eat, our level of stress, the degree to which we exercise, and many other lifestyle modifiable factors completely under our control. Why else, for instance, would smokers observe their biological age&nbsp;<a href="/blog/skin-age-reversed-13-years-just-9-months-doing1" rel="dofollow" target="_blank">visibly reverse 13 years after only 9 months of smoking cessation</a>?</em></strong></span></p><p><a href="https://greenmedinfo.com/blog/2-skin-aging-reversal-remedies-proven-clinically" target="_blank">read more</a></p> https://greenmedinfo.com/blog/2-skin-aging-reversal-remedies-proven-clinically#comments Aging Aging Skin Aging: Brain Aging: Heart Astaxanthin Neurodegenerative Diseases Rose Hips anti-inflammatory Antioxidant Health Guide: Farm/Pharma Face Off anti aging natural health natural skin care Wed, 22 Nov 2023 14:03:50 +0000 Sayer Ji 283767 at https://greenmedinfo.com 2.4 GHz electromagnetic field exposure increases DNA oxidation in brain and plasma and plasma protein oxidation in rats. Garlic reduces biomarkers of both DNA and protein oxidation, suggesting it protects against the adverse effects of EMF radiation. https://greenmedinfo.com/article/24-ghz-electromagnetic-field-exposure-increases-dna-oxidation-brain-and-plasma PMID:  Int J Radiat Biol. 2014 Oct ;90(10):892-6. Epub 2014 Jun 25. PMID: 24844368 Abstract Title:  Increased DNA oxidation (8-OHdG) and protein oxidation (AOPP) by low level electromagnetic field (2.45 GHz) in rat brain and protective effect of garlic. Abstract:  PURPOSE: To investigate the oxidative damage and protective effect of garlic on rats exposed to low level of electromagnetic fields (EMF) at 2.45 GHz Microwave radiation (MWR).METHODS: Thirty-six Wistar rats were divided into three groups. Group I was the control group and not exposed to EMF. Group II and III were exposed to low level EMF (3.68± 0.36 V/m) at 2.45 GHz MWR for 1 hour/day for 30 consecutive days. Daily 500 mg/kg garlic was given to Group III during the study period. At the end of the study, thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP) and 8-hydroxydeoxyguanosine (8-OHdG) levelswere investigated in brain tissue and blood samples.RESULTS: Exposure to low level of EMF increased 8-OHdG level in both plasma and brain tissue whereas it increased AOPP level only in plasma. Garlic prevented the increase of 8-OHdG level in brain tissue and plasma AOPP levels.CONCLUSIONS: It may be concluded that low level EMF at 2.45 GHz MWR increases the DNA damage in both brain tissues and plasma of the rats whereas it increases protein oxidation only in plasma. It may also be argued that the use of garlic decreases these effects. <p><a href="https://greenmedinfo.com/article/24-ghz-electromagnetic-field-exposure-increases-dna-oxidation-brain-and-plasma" target="_blank">read more</a></p> https://greenmedinfo.com/article/24-ghz-electromagnetic-field-exposure-increases-dna-oxidation-brain-and-plasma#comments DNA damage Garlic Oxidative Stress Antioxidant Electromagnetic Field Harms Electromagnetic Radiation Electromagnetic Radiation: 2.4 GHz Genoprotective Genotoxic Microwave Radiation Mobile Phone Radiation Oxidant Radioprotective Cell Phones electromagnetic fields electromagnetic frequencies electromagnetic radiation EMF Plant Extracts Radio Frequency Electromagnetic Radiation Exposure Animal Study Fri, 02 Aug 2019 23:38:27 +0000 greenmedinfo 192711 at https://greenmedinfo.com 50 days of exposure to 3 MT electromagnetic field induces oxidative stress and lipid peroxidation in rats, which is attentuated by vitamin E. https://greenmedinfo.com/article/50-days-exposure-3-mt-electromagnetic-field-induces-oxidative-stress-and-lipid PMID:  Basic Clin Neurosci. 2016 Oct ;7(4):315-322. PMID: 27872692 Abstract Title:  Protective Effects of Vitamin E Consumption against 3MT Electromagnetic Field Effects on Oxidative Parameters in Substantia Nigra in Rats. Abstract:  INTRODUCTION: Electromagnetic fields (EMFs) can influence the biological system by the formation of free radicals in cells. The EMFs are able to deteriorate defense system against free radicals that leads to oxidative stress (OS). Lipid peroxidation process (LPO) is an index of oxidative stress, and the Malandialdehyde (MDA) is the final product of LPO. Vitamin E is the most important antioxidant which inhibits the LPO process. The aim of this study was to evaluate the effects of 3MT EMF exposure on oxidative stress parameters in substantia nigra and the role of vitamin E in reducing oxidative stress and preventing of LPO process.METHODS: 40 male Wistar rats were randomly divided into 4 groups: 1) Control group: received standard food without exposure to EMF and without consumption of vitamin E, 2) Experimental group 1: was exposed to EMF (3MT) 4 h/day for 50 days, 3) The experimental group 2: received 200 mg/kg vitamin E with gavage every day and also was exposed to EMF (3MT) 4 h/day for 50 days, 4) Sham group: received water with gavage for 50 days.RESULTS: A significant increase in MDA levels and Glutation peroxidase (GSH-Px) activity of the substantia nigra following 50 days exposure to EMF was detected, but the superoxide dismutase (SOD) activity was decreased. Exposure did not change total antioxidant capacity (TAC) levels in plasma. Vitamin E treatment significantly prevented the increase of the MDA levels and GSHPx activity and also prevented the decrease of SOD activity in tissue but did not alter TAC levels. The GSH-Px activity increased because the duration and intensity of exposure were not enough to decrease it.CONCLUSION: We demonstrated two important findings; that 50 days exposure to 3 MT electromagnetic field caused oxidative stress by increasing the levels of MDA, and decreasing SOD activity in the substantia nigra; and that treatment with the vitamin E significantly prevented the oxidative stress and lipid peroxidation. <p><a href="https://greenmedinfo.com/article/50-days-exposure-3-mt-electromagnetic-field-induces-oxidative-stress-and-lipid" target="_blank">read more</a></p> https://greenmedinfo.com/article/50-days-exposure-3-mt-electromagnetic-field-induces-oxidative-stress-and-lipid#comments Lipid Peroxidation Oxidative Stress Vitamin E Antioxidant Electromagnetic Field Harms Electromagnetic Radiation Malondialdehyde (MDA) Up-Regulation Oxidant Superoxide Dismutase Downregulation Animal Study Mon, 04 Nov 2019 19:13:50 +0000 greenmedinfo 200910 at https://greenmedinfo.com 6 Proven Reasons to Eat the OTHER Black Berry https://greenmedinfo.com/blog/6-proven-reasons-eat-other-black-berry <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2019<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="6 Proven Reasons To Eat The OTHER Black Berry" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/Sayer Ji/images/black_raspberries_medicinal_greenmedinfo(1).jpg" style="width: 600px; height: 400px;" /></p> <p><span style="font-size:18px;"><strong><em>Chances are you can find fresh blackberries pretty easily in your local market. But black raspberries (Rubus occidentalis) are a different story. And they are worth seeking out</em></strong></span></p> <p>All berries are good sources of antioxidants but black raspberries take top prize. According to Oregan State University Department of Food Science and Technology they contain almost three times the antioxidants of blackberries and more than six times the antioxidants of red raspberries.</p> <p>They are also extremely high in anthocyanins, the antioxidant compounds that give purple foods their rich, unique color. And they are rich in ellagic acid, a powerful anti-cancer, anti-viral, and anti-bacterial compound.</p><p><a href="https://greenmedinfo.com/blog/6-proven-reasons-eat-other-black-berry" target="_blank">read more</a></p> https://greenmedinfo.com/blog/6-proven-reasons-eat-other-black-berry#comments Barrett Esophagus Berries: All Black Raspberry Black Raspberry Seed Colorectal Cancer Diabetes Mellitus: Type 2: Prevention Hypertension Oral Cancer Antioxidant Health Guides: Healing Foods Diabetes healing foods raspberries Wed, 30 Oct 2019 11:13:10 +0000 GMI Research Group 136343 at https://greenmedinfo.com Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion. https://greenmedinfo.com/article/antioxidants-inhibit-cell-senescence-and-preserve-stemness-adipose-tissue-deri PMID:  Stem Cell Res Ther. 2019 10 17 ;10(1):306. Epub 2019 Oct 17. PMID: 31623678 Abstract Title:  Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion. Abstract:  BACKGROUND: Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising candidates for regenerative medicine. However, long-term in vitro passaging leads to stemness loss and cell senescence of ADSCs, resulting in failure of ADSC-based therapy.METHODS: In this study, ADSCs were treated with low dose of antioxidants (reduced glutathione and melatonin) with anti-aging and stem cell protection properties in the in vitro passaging, and the cell functions including stem cell senescence, cell migration, cell multidirectional differentiation potential, and ROS content were carefully analyzed.RESULTS: We found that GSH and melatonin could maintain ADSC cell functions through reducing cell senescence and promoting cell migration, as well as by preserving stemness and multidirectional differentiation potential, through inhibiting ROS generation during long-term expansion of ADSCs.CONCLUSIONS: Our results suggested that antioxidant treatment could efficiently prevent the dysfunction and preserve cell functions of ADSCs after long-term passaging, providing a practical strategy to facilitate ADSC-based therapy. <p><a href="https://greenmedinfo.com/article/antioxidants-inhibit-cell-senescence-and-preserve-stemness-adipose-tissue-deri" target="_blank">read more</a></p> https://greenmedinfo.com/article/antioxidants-inhibit-cell-senescence-and-preserve-stemness-adipose-tissue-deri#comments Antioxidant formulas Glutathione Melatonin Antioxidant Regenerative Human In Vitro Mon, 24 Feb 2020 16:14:48 +0000 greenmedinfo 213457 at https://greenmedinfo.com Aqueous extract of Glycyrrhiza inflata inhibits aggregation by upregulating PPARGC1A and NFE2L2-ARE pathways in cell models of spinocerebellar ataxia 3. https://greenmedinfo.com/article/aqueous-extract-glycyrrhiza-inflata-inhibits-aggregation-upregulating-ppargc1a PMID:  Free Radic Biol Med. 2014 Jun ;71:339-350. Epub 2014 Mar 25. PMID: 24675225 Abstract Title:  Aqueous extract of Glycyrrhiza inflata inhibits aggregation by upregulating PPARGC1A and NFE2L2-ARE pathways in cell models of spinocerebellar ataxia 3. Abstract:  Spinocerebellar ataxia (SCA) types 1, 2, 3, 6, 7, and 17 and dentatorubropallidoluysian atrophy, as well as Huntington disease, are a group of neurodegenerative disorders caused by a CAG triplet-repeat expansion encoding a long polyglutamine (polyQ) tract in the respective mutant proteins. The cytoplasmic and nuclear aggregate formation, a pathological hallmark of polyQ diseases, is probably the initial process triggering the subsequent pathological events. Compromised oxidative stress defense capacity and mitochondrial dysfunction have emerged as contributing factors to the pathogenesis of polyQ diseases. The roots of licorice (Glycyrrhiza species) have long been used as an herbal medicine. In this study, we demonstrate the aggregate-inhibitory effect of Glycyrrhiza inflata herb extract and its constituents licochalcone A and ammonium glycyrrhizinate (AMGZ) in both 293 and SH-SY5Y ATXN3/Q75 cells, SCA3 cell models. The reporter assay showed that G. inflata herb extract, licochalcone A, and AMGZ could enhance the promoter activity of peroxisome proliferator-activated receptorγ, coactivator 1α (PPARGC1A), a known regulator of mitochondrial biogenesis and antioxidative response genes. G. inflata extract, licochalcone A, and AMGZ upregulated PPARGC1A expression and its downstream target genes, SOD2 and CYCS, in the 293 ATXN3/Q75 cell model. The expression of nuclear factor erythroid 2-related factor 2 (NFE2L2), the principal transcription factor that binds to antioxidant-responsive elements (AREs) to promote ARE-dependent gene expression when the cells respond to oxidative stress, and its downstream genes, HMOX1, NQO1, GCLC, and GSTP1, was also increased by G. inflata herb extract, licochalcone A, and AMGZ. Knockdown of PPARGC1A increased aggregates in ATXN3/Q75 cells and also attenuated the aggregate-inhibiting effect of the tested compounds. G. inflata extract and its constituents significantly elevated GSH/GSSG ratio and reduced reactive oxidative speciesin ATXN3/Q75 cells. The study results suggest that the tested agents activate PPARGC1A activity and NFE2L2-ARE signaling to increase mitochondrial biogenesis, decrease oxidative stress, and reduce aggregate formation in SCA3 cellular models. <p><a href="https://greenmedinfo.com/article/aqueous-extract-glycyrrhiza-inflata-inhibits-aggregation-upregulating-ppargc1a" target="_blank">read more</a></p> https://greenmedinfo.com/article/aqueous-extract-glycyrrhiza-inflata-inhibits-aggregation-upregulating-ppargc1a#comments Huntington Disease Licorice Oxidative Stress Antioxidant Neuroprotective Agents Superoxide Dismutase Up-regulation Plant Extracts In Vitro Study Thu, 20 Oct 2022 18:01:00 +0000 greenmedinfo 265141 at https://greenmedinfo.com Astaxanthin and resveratrol may have therapeutic effects against ischemia-reperfusion injury. https://greenmedinfo.com/article/astaxanthin-and-resveratrol-may-have-therapeutic-effects-against-ischemia-repe PMID:  Am J Perinatol. 2022 May 29. Epub 2022 May 29. PMID: 35644129 Abstract Title:  Therapeutic Role of Astaxanthin and Resveratrol in an Experimental Rat Model of Supraceliac Aortic Ischemia-Reperfusion. Abstract:  OBJECTIVE:  The aim of the study is to investigate the therapeutic effects of astaxanthin (AST) and resveratrol (RVT) on multiorgan damage in an animal model of the supraceliac aortic ischemia-reperfusion (I/R).METHODS:  In this study, 28 rats ( = 7/group), 200 to 250 g in weight, were randomized to four groups (1: Sham, 2: Control + I/R, 3: AST + I/R, and 4: RVT + I/R). Following the abdominal incision, aortic dissection was performed in the sham group without injury. Other groups underwent I/R injury via supraceliac aortic clamping (20 minutes) and reperfusion. The rats were administered olive oil (3 mL/kg) orally for 2 weeks before and 1 week after the laparotomy. Additionally, oral AST (10 mg/kg) or RVT (50 mg/kg) was given to the study groups. All rats were sacrificed on the 3rd week of the experiment after blood samples were taken for analysis. Multiple rat tissues were removed.RESULTS:  We found that RVT increased total antioxidant status (TAS) and superoxide dismutase (SOD) levels, and decreased total oxidant status (TOS), oxidative stress index (OSI), myeloperoxidase (MPO), and malondialdehyde (MDA) levels, while AST increased the levels of TAS, decreased TNF-α, MDA, TOS, andOSI (<p><a href="https://greenmedinfo.com/article/astaxanthin-and-resveratrol-may-have-therapeutic-effects-against-ischemia-repe" target="_blank">read more</a></p> https://greenmedinfo.com/article/astaxanthin-and-resveratrol-may-have-therapeutic-effects-against-ischemia-repe#comments Astaxanthin Ischemia Resveratrol Anti-Inflammatory Agents Antioxidant Malondialdehyde Down-regulation Tumor Necrosis Factor (TNF) Alpha Inhibitor Animal Study Sun, 12 Jun 2022 17:49:19 +0000 greenmedinfo 259001 at https://greenmedinfo.com Beneficial effects of natural phenolics on levodopa methylation and oxidative neurodegeneration. https://greenmedinfo.com/article/beneficial-effects-natural-phenolics-levodopa-methylation-and-oxidative-neurod PMID:  Brain Res. 2013 Feb 25 ;1497:1-14. Epub 2012 Dec 1. PMID: 23206800 Abstract Title:  Beneficial effects of natural phenolics on levodopa methylation and oxidative neurodegeneration. Abstract:  Levodopa (L-DOPA) is widely used for symptomatic management in Parkinson&#039;s disease. We recently showed that (-)-epigallocatechin-3-gallate, a tea polyphenol, not only inhibits L-DOPA methylation, but also protects against oxidative hippocampal neurodegeneration. In the present study, we sought to determine several other common dietary phenolics, namely, tea catechins [(+)-catechin and (-)-epicatechin] and a representative flavonoid (quercetin), for their ability to modulate L-DOPA methylation and to protect against oxidative hippocampal injury. A combination of in vitro biochemical assays, cell culture-based mechanistic analyses, and in vivo animal models was used. While both tea catechins and quercetin strongly inhibit human liver catechol-O-methyltransferase (COMT)-mediated O-methylation of L-DOPA in vitro, only (+)-catechin exerts a significant inhibition of L-DOPA methylation in both peripheral compartment and striatum in rats. The stronger in vivo effect of (+)-catechin on L-DOPA methylation compared to the other dietary compounds is due to its better bioavailability in vivo. In addition, (+)-catechin strongly reduces glutamate-induced oxidative cytotoxicity in HT22 mouse hippocampal neurons in vitro through inactivation of the nuclear factor-κB signaling pathway. Administration of (+)-catechin also exerts a strong neuroprotective effect in the kainic acid-induced oxidative hippocampal neurodegeneration model in rats. In conclusion, (+)-catechin is a dietary polyphenolic that may have beneficial effects in L-DOPA-based treatment of Parkinson patients by inhibiting L-DOPA methylation plus reducing oxidative neurodegeneration. <p><a href="https://greenmedinfo.com/article/beneficial-effects-natural-phenolics-levodopa-methylation-and-oxidative-neurod" target="_blank">read more</a></p> https://greenmedinfo.com/article/beneficial-effects-natural-phenolics-levodopa-methylation-and-oxidative-neurod#comments Catechin Neurodegenerative Diseases Tyrosine Antioxidant Excitatory Amino Acid Agonists Regenerative Nerve Regeneration Animal Study Thu, 20 Feb 2020 15:38:16 +0000 greenmedinfo 212668 at https://greenmedinfo.com Coconut oil protects against light-induced retina degeneration in male Wistar rats. https://greenmedinfo.com/article/coconut-oil-protects-against-light-induced-retina-degeneration-male-wistar-rat PMID:  Pathophysiology. 2018 Oct 5. Epub 2018 Oct 5. PMID: 30309759 Abstract Title:  Coconut oil protects against light-induced retina degeneration in male Wistar rats. Abstract:  The retinoprotective effect of Cocos nucifera oil (CNO) was investigated. Twenty male Wistar rats weighing 140 g and 180 g were randomly divided into four groups comprising of five animals each. The control group received distilled water. Retinal degeneration was induced in the remaining three groups by exposing the animals to 5,000 lux of bright white light for two hours. Prior to the light exposure,the light model group (LMG) received distilled water for 14 days, low Cocos nucifera oil (LCNO) group received 5 ml/kg of CNO for 14 days, and the high Cocos nucifera oil (HCNO) group received 10 ml/kg of CNO for 14 days. The treatments continued for 7 days after exposure to light. On the eightday, the animals were euthanised and their retinas isolated. The right retinas and occipital cortices of the animals were prepared for histological evaluation while the homogenates of the left retinas were used for biochemical assay. The results show that CNO significantly (p <p><a href="https://greenmedinfo.com/article/coconut-oil-protects-against-light-induced-retina-degeneration-male-wistar-rat" target="_blank">read more</a></p> https://greenmedinfo.com/article/coconut-oil-protects-against-light-induced-retina-degeneration-male-wistar-rat#comments Coconut Oil Retinal Degeneration: Light-Induced Anti-Apoptotic Antioxidant Animal Study Mon, 29 Oct 2018 01:42:34 +0000 greenmedinfo 173119 at https://greenmedinfo.com Cordyceps supplementation prevents adverse effects of electromagnetic pulse irradiation on sperm quality. https://greenmedinfo.com/article/cordyceps-supplementation-prevents-adverse-effects-electromagnetic-pulse-irrad PMID:  OMICS. 2017 02 ;21(2):81-89. PMID: 28186865 Abstract Title:  Preventing Electromagnetic Pulse Irradiation Damage on Testis Using Selenium-rich Cordyceps Fungi. A Preclinical Study in Young Male Mice. Abstract:  Networked 21st century society, globalization, and communications technologies are paralleled by the rise of electromagnetic energy intensity in our environments and the growing pressure of the environtome on human biology and health. The latter is the entire complement of environmental factors, including the electromagnetic energy and the technologies that generate them, enacting on the digital citizen in the new century. Electromagnetic pulse (EMP) irradiation might have serious damaging effects not only on electronic equipment but also in the whole organism and reproductive health, through nonthermal effects and oxidative stress. We sought to determine whether EMP exposure (1) induces biological damage on reproductive health and (2) the extent to which selenium-rich Cordyceps fungi (daily coadministration) offer protection on the testicles and spermatozoa. In a preclinical randomized study, 3-week-old male BALB/c mice were repeatedly exposed to EMP (peak intensity 200 kV/m, pulse edge 3.5 ns, pulse width 15 ns, 0.1 Hz, and 400 pulses/day) 5 days per week for four consecutive weeks, with or without coadministration of daily selenium-rich Cordyceps fungi (100 mg/kg). Testicular index and spermatozoa formation were measured at baseline and 1, 7, 14, 28, and 60 day time points after EMP exposure. The group without Cordyceps cotreatment displayed decreased spermatozoa formation, shrunk seminiferous tubule diameters, and diminished antioxidative capacity at 28 and 60 days after exposure (p <p><a href="https://greenmedinfo.com/article/cordyceps-supplementation-prevents-adverse-effects-electromagnetic-pulse-irrad" target="_blank">read more</a></p> https://greenmedinfo.com/article/cordyceps-supplementation-prevents-adverse-effects-electromagnetic-pulse-irrad#comments Oxidative Stress Selenium Sperm Count: Low Sperm Quality: Low Anti-Apoptotic Antioxidant Cytoprotective Electromagnetic Field Harms Electromagnetic Radiation Animal Study Mon, 28 Oct 2019 17:27:15 +0000 greenmedinfo 200295 at https://greenmedinfo.com Curcuma aromatica water extract attenuates ethanol-induced gastritis via enhancement of antioxidant status. https://greenmedinfo.com/article/curcuma-aromatica-water-extract-attenuates-ethanol-induced-gastritis-enhanceme PMID:  Evid Based Complement Alternat Med. 2015 ;2015:582496. Epub 2015 Sep 21. PMID: 26483844 Abstract Title:  Curcuma aromatica Water Extract Attenuates Ethanol-Induced Gastritis via Enhancement of Antioxidant Status. Abstract:  Curcuma aromatica is an herbal medicine and traditionally used for the treatment of various diseases in Asia. We investigated the effects of C. aromatica water extract (CAW) in the stomach of rats with ethanol-induced gastritis. Gastritis was induced in rats by intragastric administration of 5 mL/kg body weight of absolute ethanol. The CAW groups were given 250 or 500 mg of extract/kg 2 h before administration of ethanol, respectively. To determine the antioxidant effects of CAW, we determined the level of lipid peroxidation, the level of reduced glutathione (GSH), the activities of catalase, degree of inflammation, and mucus production in the stomach. CAW reduced ethanol-induced inflammation and loss of epithelial cells and increased the mucus production in the stomach. CAW reduced the increase in lipid peroxidation associated with ethanol-induced gastritis (250 and 500 mg/kg, p<p><a href="https://greenmedinfo.com/article/curcuma-aromatica-water-extract-attenuates-ethanol-induced-gastritis-enhanceme" target="_blank">read more</a></p> https://greenmedinfo.com/article/curcuma-aromatica-water-extract-attenuates-ethanol-induced-gastritis-enhanceme#comments Alcohol Toxicity Gastritis Turmeric Antioxidant Animal Study Wed, 13 Jul 2022 22:52:47 +0000 greenmedinfo 260414 at https://greenmedinfo.com Dietary supplementation of barley and/or dates attenuate hypercholesterolemic-induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic pathways and enhancing oxidative capacity. https://greenmedinfo.com/article/dietary-supplementation-barley-andor-dates-attenuate-hypercholesterolemic-indu PMID:  J Food Biochem. 2019 Nov ;43(11):e13001. Epub 2019 Aug 1. PMID: 31373029 Abstract Title:  Dietary supplementation of barley and/or dates attenuate hypercholesterolemic-induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic pathways and enhancing oxidative capacity. Abstract:  Our study was conducted to characterize the efficacy of barley and/or date palm fruits (10%) in alleviation of hypercholesterolemic endometrial insults in obese rat model. Sixty-four Wistar albino rats were randomized into eight groups (n = 8); control, hypercholesterolemic- and hypercholesterolemic-treated groups. Animals were subjected to treatment for 4 months. After sacrifice, serum and uterine tissues were collected and processed for biochemical, histological, immunohistochemical, and electron microscopic investigations. Inhypercholesterolemic rats, the endometrium displayed hyperplasia with necrotic patches in the surface epithelium and its glandular lining cells. Also, there was a remarkable increase in the endometrial thickness and significant decrease in corresponding glandular numbers. Prompted by these findings,immunohistochemical localization revealed that expression of proliferating cell nuclear antigen was downregulated, while cleaved caspase-3 was upregulated in the endometrial cells in hypercholesterolemic group. Accordingly, there was remarkable depletion of antioxidant enzymatic activities associated with increased lipid peroxidation and apoptotic markers. Contradictory, supplementation of barley and/or dates to hypercholesterolemic groups showed intriguing amelioration for the histological architecture of the endometrium and balancing its oxidative redox. In conclusion, the administration ofbarley and/or dates confers enhanced synergistic effects in attenuation of hypercholesterolemic induced-endometrial dysfunction. This is clear evidence that endometrial amelioration was directly linked to the implication of highly potential antioxidant capacity of barley and/or dates phytochemicals, β-glucan, polyphenols, and other trace elements, which can be utilized to establish a phyto-therapeutic strategy for activating endometrial cell regeneration. PRACTICAL APPLICATIONS: Barley and dates confer both hypoglycemic and hypocholesterolemic potentials. Therefore, their ingredients would be implicated in the amelioration of uterine functions in obese women. These favorable potentials were directly linked to the restraining of endometrial inflammation and retrieving the oxidative capacity. Furthermore, our findings demonstrated that barley and dates substantially diminished the expression of TNF-α, mitigated DNA damage and prevented leukocytic infiltration in the endometrial tissue; based on their high content of dietary phytochemicals, β-glucan, polyphenols, and other trace elements. <p><a href="https://greenmedinfo.com/article/dietary-supplementation-barley-andor-dates-attenuate-hypercholesterolemic-indu" target="_blank">read more</a></p> https://greenmedinfo.com/article/dietary-supplementation-barley-andor-dates-attenuate-hypercholesterolemic-indu#comments Barley Dates Hypercholesterolemia Oxidative Stress Anti-Apoptotic Antioxidant Animal Study Fri, 28 Feb 2020 20:19:54 +0000 greenmedinfo 214515 at https://greenmedinfo.com Diosgenin inhibits telomerase activity by down regulation of hTERT gene expression in A549 cell line. https://greenmedinfo.com/article/diosgenin-inhibits-telomerase-activity-down-regulation-htert-gene-expression-a PMID:  Asian Pac J Cancer Prev. 2013 ;14(11):6945-8. PMID: 24377630 Abstract Title:  Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line. Abstract:  BACKGROUND: Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target.MATERIALS AND METHODS: We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively.RESULTS: MTT results showed that IC50 values for 24, 48 and 72h after treatment were 47, 44 and 43μM, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression.DISCUSSION: These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line. https://greenmedinfo.com/article/diosgenin-inhibits-telomerase-activity-down-regulation-htert-gene-expression-a#comments Diosgenin Fenugreek Lung Cancer Antioxidant Antiproliferative Chemopreventive Telomerase Inhibitor Gene Expression Regulation In Vitro Study Sun, 29 Jun 2014 22:47:40 +0000 greenmedinfo 113068 at https://greenmedinfo.com Effects of astaxanthin supplementation in healthy and obese dogs. https://greenmedinfo.com/article/effects-astaxanthin-supplementation-healthy-and-obese-dogs PMID:  Vet Med (Auckl). 2019 ;10:29-35. Epub 2019 Feb 15. PMID: 30859086 Abstract Title:  Effects of astaxanthin supplementation in healthy and obese dogs. Abstract:  Background: Since astaxanthin (ASX) has potent anti-oxidative effects with inhibitory action of lipid peroxidation and singlet oxygen quenching activity, it is widely used as a functional food for keeping good health in human. Obesity is a risk factor for various metabolic disorders. It is characterized by low-grade chronic inflammation based on oxidative stress by excessively produced ROS. From the point of preventive medicine, natural compounds have been proposed as potential therapeutic agents in the prevention of metabolic disorder in companion animals. The purpose of this study is to evaluate the effects of ASX supplementation in healthy and obese dogs.Materials and methods: Ten healthy beagle dogs and 5 clinically obese dogs were used in this study. The healthy beagle dogs were randomly divided into 2 groups as follows: control and test groups. The test group dogs received ASX supplementation mixed with the food for 6 weeks. Five clinically obese dogs received ASX supplementation for 8 weeks. Metabolites, hormones and enzymes were measured before and after ASX supplementation.Results: In the healthy dog groups, after 6 weeks, plasma triglyceride (TG) and malondialdehyde concentrations and lactate dehydrogenase (LDH) values significantly decreased in the test group. There was no significant difference in the control group. In clinically obese dogs, plasma TG concentration decreased after 8 weeks of ASX supplementation. Plasma alanine aminotransferase and LDH values clearly decreased in all 5 dogs and 4 dogs out of 5 dogs, respectively.Conclusion: ASX supplementation (0.3 mg/kg body weight/day) for 6 weeks in healthy dogs and 8 weeks in obese dogs induced the elevation of antioxidant function and of liver function by ameliorating lipid metabolism. <p><a href="https://greenmedinfo.com/article/effects-astaxanthin-supplementation-healthy-and-obese-dogs" target="_blank">read more</a></p> https://greenmedinfo.com/article/effects-astaxanthin-supplementation-healthy-and-obese-dogs#comments Astaxanthin Obesity Antioxidant Hepatoprotective Animal Study Fri, 15 Mar 2019 01:04:27 +0000 greenmedinfo 181359 at https://greenmedinfo.com