Glioblastoma https://greenmedinfo.com/category/keywords/Glioblastoma en Cancer as a Curable Metabolic Disease https://greenmedinfo.com/blog/cancer-curable-metabolic-disease <p class="rtecenter"><span style="font-size:14px;"><span style="font-family:verdana,geneva,sans-serif;"><strong><span style="color: rgb(34, 34, 34); background-color: rgb(255, 255, 255);">This article is copyrighted by Jeffrey Dach MD, 2013</span><br style="color: rgb(34, 34, 34); font-family: &quot;Helvetica Neue&quot;, Helvetica, Arial, sans-serif; font-size: 13px; background-color: rgb(255, 255, 255);" /> <span style="color: rgb(34, 34, 34); background-color: rgb(255, 255, 255);">Republished with permission from <a href="https://jeffreydachmd.com/2015/01/cancer-metabolic-disease-jeffrey-dach-md/" rel="dofollow" target="_blank">JeffreyDachMD.com</a></span></strong></span></span></p> <p class="rtecenter"><img alt="Cancer as a Metabolic Disease" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/Sayer Ji/images/cancer_metabolic_disease.jpg" /></p><p><a href="https://greenmedinfo.com/blog/cancer-curable-metabolic-disease" target="_blank">read more</a></p> https://greenmedinfo.com/blog/cancer-curable-metabolic-disease#comments Berberine Betulinic acid Breast Cancer Cancer Curcumin Glioblastoma Grapes Lung Cancer Prostate Cancer Pterostilbene Resveratrol Vitamin K Chemotherapy Berberine Betulinic acid Breast Cancer Cancer Chemotherapy CURCUMIN Glioblastoma Grapes lung cancer prostate cancer Pterostilbene RESVERATROL Vitamin K Wed, 14 Jan 2015 20:23:49 +0000 drdach 115995 at https://greenmedinfo.com Combined use of lower concentrations of jerantinine A and γ-tocotrienol induced potent cytotoxic effects on U87MG cancer cells. https://greenmedinfo.com/article/combined-use-lower-concentrations-jerantinine-and-tocotrienol-induced-potent-c n/a PMID:  Phytomedicine. 2017 Jul 1 ;30:74-84. Epub 2017 Mar 10. PMID: 28545672 Abstract Title:  Enhancement of apoptotic activities on brain cancer cells via the combination ofγ-tocotrienol and jerantinine A. Abstract:  BACKGROUND: γ-Tocotrienol, a vitamin E isomer possesses pronounced in vitro anticancer activities. However, the in vivo potency has been limited by hardly achievable therapeutic levels owing to inefficient high-dose oral delivery which leads to subsequent metabolic degradation. Jerantinine A, an Aspidosperma alkaloid, originally isolated from Tabernaemontana corymbosa, has proved to possess interesting anticancer activities. However, jerantinine A also induces toxicity to non-cancerous cells. PURPOSE: We adopted a combinatorial approach with the joint application ofγ-tocotrienol and jerantinine A at lower concentrations in order to minimize toxicity towards non-cancerous cells while improving the potency on brain cancer cells. METHODS: The antiproliferative potency of individualγ-tocotrienol and jerantinine A as well as combined in low-concentration was firstly evaluated on U87MG cancer and MRC5 normal cells. Morphological changes, DNA damage patterns, cell cycle arrests and the effects of individual and combined low-concentration compounds on microtubules were then investigated. Finally, the potential roles of caspase enzymes and apoptosis-related proteins in mediating the apoptotic mechanisms were investigated using apoptosis antibody array, ELISA and Western blotting analysis. RESULTS: Combinatorial study betweenγ-tocotrienol at a concentration range (0-24µg/ml) and fixed IC20 concentration of jerantinine A (0.16µg/ml) induced a potent antiproliferative effect on U87MG cells and led to a reduction on the new half maximal inhibitory concentration of γ-tocotrienol (i.e.tIC50=1.29µg/ml) as compared to that of individual γ-tocotrienol (i.e. IC50=3.17µg/ml). A reduction on undesirable toxicity to MRC5 normal cells was also observed. G0/G1 cell cycle arrest was evident on U87MG cells receiving IC50 of individual γ-tocotrienol and combined low-concentration compounds (1.29µg/ml γ-tocotrienol + 0.16µg/ml jerantinine A), whereas, a profound G2/M arrest was evident on cells treated with IC50 of individual jerantinine A. Additionally, individual jerantinine A and combined compounds (except individual γ-tocotrienol) caused a disruption of microtubule networks triggering Fas- and p53-induced apoptosis mediated via the death receptor and mitochondrial pathways. CONCLUSIONS: These findings demonstrated that the combined use of lower concentrations ofγ-tocotrienol and jerantinine A induced potent cytotoxic effects on U87MG cancer cells resulting in a reduction on the required individual concentrations and thereby minimizing toxicity of jerantinine A towards non-cancerous MRC5 cells as well as probably overcoming the high-dose limiting application of γ-tocotrienol. The multi-targeted mechanisms of action of the combination approach have shown a therapeutic potential against brain cancer in vitro and therefore, further in vivo investigations using a suitable animal model should be the way forward. https://greenmedinfo.com/article/combined-use-lower-concentrations-jerantinine-and-tocotrienol-induced-potent-c#comments Glioblastoma Vitamin E: Gamma-tocotrienol Antiproliferative Apoptotic Cell cycle arrest Chemoprotective Agents Chemosensitizer Chemotherapeutic Tumor Suppressor Protein p53 Upregulation Antiproliferative Apoptotic Cell cycle arrest Glioblastoma Natural Substance/Drug Synergy Vitamin E: Gamma-tocotrienol In Vitro Study Wed, 21 Jun 2017 02:51:54 +0000 greenmedinfo 149383 at https://greenmedinfo.com Curcumin significantly decreased glioblastoma stem cells proliferation, sphere forming ability and colony forming potential. https://greenmedinfo.com/article/curcumin-significantly-decreased-glioblastoma-stem-cells-proliferation-sphere- n/a PMID:  BMC Cancer. 2017 Feb 4 ;17(1):99. Epub 2017 Feb 4. PMID: 28160777 Abstract Title:  Curcumin decreases malignant characteristics of glioblastoma stem cells via induction of reactive oxygen species. Abstract:  BACKGROUND: Glioblastoma Multiforme (GBM) is the most common and lethal form of primary brain tumor in adults. Following standard treatment of surgery, radiation and chemotherapy, patients are expected to survive 12-14 months. Theorized cause of disease recurrence in these patients is tumor cell repopulation through the proliferation of treatment-resistant cancer stem cells. Current research has revealed curcumin, the principal ingredient in turmeric, can modulate multiple signaling pathways important for cancer stem cell self-renewal and survival. METHODS: Following resection, tumor specimens were dissociated and glioblastoma stem cells (GSCs) were propagated in neurosphere media and characterized via immunocytochemistry. Cell viability was determined with MTS assay. GSC proliferation, sphere forming and colony forming assays were conducted through standard counting methods. Reactive oxygen species (ROS) production was examined using the fluorescent molecular probe CM-H2DCFA. Effects on cell signaling pathways were elucidated by western blot. RESULTS: We evaluate the effects of curcumin on patient-derived GSC lines. We demonstrate a curcumin-induced dose-dependent decrease in GSC viability with an approximate IC50 of 25 μM. Treatment with sub-toxic levels (2.5 μM) of curcumin significantly decreased GSC proliferation, sphere forming ability and colony forming potential. Curcumin induced ROS, promoted MAPK pathway activation, downregulated STAT3 activity and IAP family members. Inhibition of ROS with the antioxidant N-acetylcysteine reversed these effects indicating a ROS dependent mechanism. CONCLUSIONS: Discoveries made in this investigation may lead to a non-toxic intervention designed to prevent recurrence in glioblastoma by targeting glioblastoma stem cells. https://greenmedinfo.com/article/curcumin-significantly-decreased-glioblastoma-stem-cells-proliferation-sphere-#comments Curcumin Glioblastoma Antiproliferative STAT3 Inhibitor Antiproliferative Cancer Stem Cells CURCUMIN Glioblastoma STAT3 Inhibitor In Vitro Study Fri, 07 Apr 2017 20:36:55 +0000 greenmedinfo 146050 at https://greenmedinfo.com Shikonin and temozolomide may constitute a powerful new tool for Glioblastoma treatment by reducing therapy resistance and tumor recurrence. https://greenmedinfo.com/article/shikonin-and-temozolomide-may-constitute-powerful-new-tool-glioblastoma-treatm n/a PMID:  Cell Oncol (Dordr). 2017 Apr 11. Epub 2017 Apr 11. PMID: 28401486 Abstract Title:  Dual treatment with shikonin and temozolomide reduces glioblastoma tumor growth, migration and glial-to-mesenchymal transition. Abstract:  PURPOSE: Glioblastomas (GBM) comprise 17% of all primary brain tumors. These tumors are extremely aggressive due to their infiltrative capacity and chemoresistance, with glial-to-mesenchymal transition (GMT) proteins playing a prominent role in tumor invasion. One compound that has recently been used to reduce the expression of these proteins is shikonin (SHK), a naphthoquinone with anti-tumor properties. Temozolomide (TMZ), the most commonly used chemotherapeutic agent in GBM treatment, has so far not been studied in combination with SHK. Here, we investigated the combined effects of these two drugs on the proliferation and motility of GBM-derived cells. METHODS: The cytotoxic and proliferative effects of SHK and TMZ on human GBM-derived cells were tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Ki67 staining and BrdU incorporation assays. The migration capacities of these cells were evaluated using a scratch wound assay. The expression levels ofβ3 integrin, metalloproteinases (MMPs) and GMT-associated proteins were determined by Western blotting and immunocytochemistry. RESULTS: We found that GBM-derived cells treated with a combination of SHK and TMZ showed decreases in their proliferation and migration capacities. These decreases were followed by the suppression of GMT through a reduction ofβ3 integrin, MMP-2, MMP-9, Slug and vimentin expression via inactivation of PI3K/AKT signaling. CONCLUSION: From our results we conclude that dual treatment with SHK and TMZ may constitute a powerful new tool for GBM treatment by reducing therapy resistance and tumor recurrence. https://greenmedinfo.com/article/shikonin-and-temozolomide-may-constitute-powerful-new-tool-glioblastoma-treatm#comments Glioblastoma Shikonin Anti-metastatic Antiproliferative Matrix metalloproteinase-2 (MMP-2) inhibitor Matrix metalloproteinase-9 (MMP-9) inhibitor Anti-metastatic Antiproliferative Chemotherapeutic Synergy: Temozolomide Glioblastoma Shikonin Human In Vitro Wed, 03 May 2017 15:01:18 +0000 greenmedinfo 147157 at https://greenmedinfo.com Tangeretin may be a therapeutic agent for glioblastoma treatment. https://greenmedinfo.com/article/tangeretin-may-be-therapeutic-agent-glioblastoma-treatment n/a PMID:  Biomed Pharmacother. 2016 Jul ;81:491-6. PMID: 27261630 Abstract Title:  Tangeretin induces cell cycle arrest and apoptosis through upregulation of PTEN expression in glioma cells. Abstract:  Tangeretin (TANG), present in peel of citrus fruits, has been shown to various medicinal properties such as chemopreventive and neuroprotective. However, the chemopreventive effect of TANG on glioblastoma cells has not been examined. The present study was designed to explore the anticancer potential of TANG in glioblastoma cells and to investigate the related mechanism. Human glioblastoma U-87MG and LN-18 cells were treated with 45μM concentration of TANG and cell growth was measured by MTT assay. The cell cycle distribution and cell death were measured by flow cytometry. The expression of cell cycle and apoptosis related genes were analyzed by quantitative RT-PCR and western blot. The cells treated with TANG were significantly increased cell growth suppression and cell death effects than vehicle treated cells. Further, TANG treatment increases G2/M arrest and apoptosis by modulating PTEN and cell-cycle regulated genes such as cyclin-D and cdc-2 mRNA and protein expressions. Moreover, the ability of TANG to decrease cell growth and to induce cell death was compromised when PTEN was knockdown by siRNA. Taken together, the chemopreventive effect of TANG is associated with regulation of cell-cycle and apoptosis in glioblastoma, thereby attenuating glioblastoma cell growth. Hence, the present findings suggest that TANG may be a therapeutic agent for glioblastoma treatment. https://greenmedinfo.com/article/tangeretin-may-be-therapeutic-agent-glioblastoma-treatment#comments Glioblastoma Tangeretin Antiproliferative Apoptotic Cell cycle arrest Antiproliferative Apoptotic Cell cycle arrest Glioblastoma Tangeretin In Vitro Study Wed, 10 May 2017 01:24:21 +0000 greenmedinfo 147524 at https://greenmedinfo.com Total extract of Curcuma longa could be regarded as being more effective against lung cancer cells in vitro than its separated compounds. https://greenmedinfo.com/article/total-extract-curcuma-longa-could-be-regarded-being-more-effective-against-lun n/a PMID:  Phytother Res. 2018 Jan 24. Epub 2018 Jan 24. PMID: 29368356 Abstract Title:  Superior anticancer activity is demonstrated by total extract of Curcuma longa L. as opposed to individual curcuminoids separated by centrifugal partition chromatography. Abstract:  Three curcuminoids: bisdemethoxycurcumin, demethoxycurcumin, and curcumin from turmeric were successfully separated by a high capacity solvent system composed of heptane: chloroform: methanol: water mixture (5: 6: 3: 2 v/v/v/v) tailored for centrifugal partition chromatographs at K-values of 0.504, 1.057, 1.644, respectively. These three ferulic acid derivatives obtained at a purity rate exceeding 95% were analysed by an HPLC-MS spectrometer. Turmeric extract inhibited the proliferation/viability of A549 human lung cancer, HT29 colon cancer, and T98G glioblastoma cell lines in (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay (MTT). Single curcuminoids significantly decreased the viability/proliferation of lung cancer cells in a dose-dependent manner. However, totalextract displayed the superior anticancer activity in the investigated cell lines. Crude extract in combination with cisplatin augmented the decrease in the viability of cancer cells compared with single compound treatment in A549 lung cancer cells. Total extract of Curcuma longa could be regardedas being more effective against lung cancer cells in vitro than its separated compounds. https://greenmedinfo.com/article/total-extract-curcuma-longa-could-be-regarded-being-more-effective-against-lun#comments Colon Cancer Glioblastoma Lung Cancer Turmeric Antiproliferative Antiproliferative COLON CANCER Glioblastoma lung cancer Turmeric Whole Plant In Vitro Study Sat, 24 Feb 2018 08:07:12 +0000 greenmedinfo 160423 at https://greenmedinfo.com