Puerarin https://greenmedinfo.com/category/keywords/Puerarin en Co-administrated puerarin and zinc can partially reverse OVX-induced bone loss. https://greenmedinfo.com/article/co-administrated-puerarin-and-zinc-can-partially-reverse-ovx-induced-bone-loss n/a PMID:  Life Sci. 2016 Dec 1 ;166:20-26. Epub 2016 Sep 30. PMID: 27697446 Abstract Title:  Coadministration of puerarin (low dose) and zinc attenuates bone loss and suppresses bone marrow adiposity in ovariectomized rats. Abstract:  AIMS: Puerarin is a phytoestrogen that shows osteogenic effects. Meanwhile, zinc stimulates bone formation and inhibits bone resorption. The study aims to investigate the effects of coadministration of puerarin (low dose) and zinc on bone formation in ovariectomized rats. MATERIALS AND METHODS: Co-administration or use alone of puerarin (low dose) and/or zinc were gavaged in OVX rats. The estrogen-like effects were detected by the uterus weight, the histologic observation and the IGF-1 protein expression. The osteogenic effects were determined by bone histomorphometric and mechanical parameters, osteogenic and adipogenic blood markers, and so on. KEY FINDINGS: The results showed that oral administration of puerarin (low dose) plus zinc didn't significantly increase uterus weight. The glandular epithelial of endometrium had no proliferation and no protein expression of IGF-1. Moreover, co-administration attenuated bone loss and biomechanical decrease more than single use of puerarin or zinc (p<0.05). Next, combined administration of puerarin and zinc promoted the serological level of osteocalcin, bone marrow stromal cell (BMSC) proliferation, and the expression of alkaline phosphatase (ALP), and suppressed the serological level of adiponectin and adiposity in bone marrow (BM). SIGNIFICANCE: In conclusion, co-administrated puerarin (low dose) and zinc can partially reverse OVX-induced bone loss and suppress the adiposity of BM in rats, which shed light on the potential use of puerarin and zinc in the treatment of osteoporosis. https://greenmedinfo.com/article/co-administrated-puerarin-and-zinc-can-partially-reverse-ovx-induced-bone-loss#comments Osteoporosis Puerarin Zinc Osteogenic Osteoprotective bone loss Osteoporosis Ovariectomy-Induced Changes Puerarin zinc Animal Study Fri, 02 Dec 2016 21:54:42 +0000 greenmedinfo 139815 at https://greenmedinfo.com Puerarin can decrease the blood glucose level of T2DM. https://greenmedinfo.com/article/puerarin-can-decrease-blood-glucose-level-t2dm n/a PMID:  Zhongguo Zhong Yao Za Zhi. 2008 Aug ;33(16):2026-8, 2060. PMID: 19086646 Abstract Title:  [Effects of puerarin on ADRP gene expression in fatty tissue of type 2 diabetes mellitus rats]. Abstract:  OBJECTIVE: To observe the effects of puerarin on ADRP gene mRNA expression in fatty tissue of type 2 diabetes mellitus rats (T2DM). METHOD: Wiastar rats of T2DM model were made by feeding with high glucose and fat diet and injecting with small dose of streptozocin (25 mg x kg(-1)). 40 model rats were randomly divided into model control group and three puerarin groups (40, 80, 160 mg x kg(-1)), another 10 rats were selected as normal control group. FBG and FINS were measured to calculate IR after rats were injected consecutively for 6 weeks. The level of ADRP gene mRNA in fatty tissue was determined by RT-PCR after rats were injected eight weeks. RESULT: Compared with model control group, high and middle dosage of puerarin can decreased ADRP gene mRNA expression in fatty tissue obviously, FBG, IR level in each puerarin group and FINS in high and middle dosage puerarin groups decreased obviously. CONCLUSION: Puerarin can decrease the blood glucose level of T2DM by downregulating ADRP mRNA expression and depressing the insulin resistance. https://greenmedinfo.com/article/puerarin-can-decrease-blood-glucose-level-t2dm#comments Diabetes Mellitus: Type 2 Puerarin Hypoglycemic Agents Diabetes mellitus: Type 2 Gene Expression Regulation Hypoglycemic Agents Puerarin Animal Study Wed, 06 Jun 2018 23:11:58 +0000 greenmedinfo 165385 at https://greenmedinfo.com Puerarin can inhibit the adhesion, invasion and migration of oophoroma HO-8910 cells. https://greenmedinfo.com/article/puerarin-can-inhibit-adhesion-invasion-and-migration-oophoroma-ho-8910-cells n/a PMID:  Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Jul ;29(7):632-5. PMID: 19852298 Abstract Title:  [Inhibitory effects of puerarin on invasion and metastasis of oophoroma cells HO-8910]. Abstract:  OBJECTIVE: To investigate the inhibitory effect of puerarin on invasive and metastatic abilities of tumor cells, and its possible mechanism through observing its impacts on the migratory, adhesive and invasive capacities of human oophoroma cells HO-8910 to the artificial recombined basement membrane. METHODS: Expression of estrogen receptor (ER) in HO-8910 cells was detected using PCR assay. Effects of puerarin on HO-8910 proliferation was detected with MTT assay; on its adhesion potential was tested with cell-matrigel adhesion assay, and on invasive and migratory capacities were measured with Transwell matrigel invasion assay and Transwell motility assay respectively. RESULTS: ER was positively expressed in HO-8910 cells. After being treated with 20 micromol/L puerarin for 12 h, the adhesive test showed that OD value in the tested group was significantly lower than that in the control (P<0.01), the inhibiting rate reached 50.63%; and the Transwell assay showed a significant lowering of penetrated cells (P<0.01), the inhibition rate for invasion was 38.59% and that for motility migration 40.63%. The number of penetrated cells was lower in the group intervened with combination of Puerarin and estrogen than in the group intervened with estrogen alone, 33.40 +/- 3.30 vs 48.05 +/- 3. 56 for invasion and 35.35 +/- 3.03 vs 52.45 +/- 1.04 for motility (all P<0.01). CONCLUSION: Puerarin can inhibit the adhesion, invasion and migration of HO-8910 cells, plays an antagonist effect against the stimulation of estrogen on the malignant behavior of tumor cells. https://greenmedinfo.com/article/puerarin-can-inhibit-adhesion-invasion-and-migration-oophoroma-ho-8910-cells#comments Cancer Metastasis Ovarian Cancer Puerarin Anti-metastatic Anti-metastatic Cancer Metastasis ovarian cancer Puerarin In Vitro Study Wed, 06 Jun 2018 23:54:03 +0000 greenmedinfo 165390 at https://greenmedinfo.com Puerarin inhibited cadmium induced apoptosis in rPT cells by ameliorating the mitochondrial dysfunction. https://greenmedinfo.com/article/puerarin-inhibited-cadmium-induced-apoptosis-rpt-cells-ameliorating-mitochondr n/a PMID:  Chem Biol Interact. 2016 Dec 25 ;260:219-231. Epub 2016 Oct 4. PMID: 27717697 Abstract Title:  Puerarin protects against cadmium-induced proximal tubular cell apoptosis by restoring mitochondrial function. Abstract:  Puerarin (PU) is a potent free radical scavenger with a protective effect in nephrotoxin-mediated oxidative damage. Here, we show a novel molecular mechanism by which PU exerts its anti-apoptotic effects in cadmium (Cd)-exposed primary rat proximal tubular (rPT) cells. Morphological assessment and flow cytometric analysis revealed that PU significantly decreased Cd-induced apoptotic cell death of rPT cells. Administration of PU protected cells against Cd-induced depletion of mitochondrial membrane potential (ΔΨm) and lipid peroxidation. Cd-mediated mitochondrial permeability transition pore (MPTP) opening, disruption of mitochondrial ultrastructure, mitochondrial cytochrome c (cyt-c) release, caspase-3 activation and subsequently poly ADP-ribose polymerase (PARP) cleavage could be effectively blockedby the addition of PU. Moreover, up-regulation of Bcl-2 and down-regulation of Bax and hence increased Bcl-2/Bax ratio were observed with the PU administration. In addition, PU reversed Cd-induced ATP depletion by restoring ΔΨm to affect ATP production and by regulating expression levels of ANT-1and ANT-2 to improve ATP transport. In summary, PU inhibited Cd-induced apoptosis in rPT cells by ameliorating the mitochondrial dysfunction. https://greenmedinfo.com/article/puerarin-inhibited-cadmium-induced-apoptosis-rpt-cells-ameliorating-mitochondr#comments Cadmium Poisoning Puerarin Anti-Apoptotic Bax/Bcl2 ratio: Increase Cadmium Anti-Apoptotic Bax/Bcl2 Cadmium Cadmium Poisoning Puerarin In Vitro Study Fri, 02 Dec 2016 21:46:48 +0000 greenmedinfo 139813 at https://greenmedinfo.com Puerarin prevents LPS-induced acute lung injury via inhibiting inflammatory response. https://greenmedinfo.com/article/puerarin-prevents-lps-induced-acute-lung-injury-inhibiting-inflammatory-respon n/a PMID:  Microb Pathog. 2018 Mar 20 ;118:170-176. Epub 2018 Mar 20. PMID: 29571724 Abstract Title:  Puerarin prevents LPS-induced acute lung injury via inhibiting inflammatory response. Abstract:  Acute lung injury (ALI) is a critical illness syndrome with high morbidity and mortality in patients. Inflammation has been known to be involved in the development of ALI. The purpose of this study was to investigate the effect of puerarin on lipopolysaccharide (LPS)-induced ALI in mice. The pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were determined by ELISA. Western blot analysis was used for detecting the expression of NF-κB, IκBα, and LXRα. And myeloperoxidase (MPO) activity, lung wet/dry (W/D) ratio, and histopathological examination were also detected in lung tissues. The results showed that puerarinsignificantly inhibited LPS-stimulated MPO activity in lung tissues. Meanwhile, puerarin attenuated lung histopathological changes and lung wet/dry (W/D) ratio. We also found that the expression of pro-inflammatory cytokines, TNF-α, IL-6 and IL-1β were inhibited by puerarin. Puerarin also inhibited LPS-induced TNF-α in RAW264.7 cells and IL-8 in A549 cells. From the results of western blotting, puerarin significantly suppressed LPS-stimulated NF-κB activation. And the expression of LXRα was dose-dependently increased by treatment of puerarin. The inhibition of puerarin on TNF-α production in RAW264.7 cells and IL-8 production in A549 cells were blocked by LXRα inhibitor geranylgeranyl pyrophosphate (GGPP). These results suggested that puerarin attenuated ALI by activating LXRα, which subsequently inhibited LPS-induced inflammatory response. https://greenmedinfo.com/article/puerarin-prevents-lps-induced-acute-lung-injury-inhibiting-inflammatory-respon#comments Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Puerarin Anti-Inflammatory Agents Tumor Necrosis Factor (TNF) Alpha Inhibitor Anti-Inflammatory Agents Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Puerarin Tumor Necrosis Factor (TNF) Alpha Inhibitor In Vitro Study Wed, 16 May 2018 19:21:43 +0000 greenmedinfo 164305 at https://greenmedinfo.com Puerarin protects against high glucose-induced acute vascular dysfunction. https://greenmedinfo.com/article/puerarin-protects-against-high-glucose-induced-acute-vascular-dysfunction n/a PMID:  Vascul Pharmacol. 2009 Mar-Apr;50(3-4):110-5. Epub 2008 Nov 24. PMID: 19073281 Abstract Title:  Puerarin protects against high glucose-induced acute vascular dysfunction: role of heme oxygenase-1 in rat thoracic aorta. Abstract:  OBJECTIVE: To investigate the antioxidant and vascular protective effect of puerarin, an isoflavone glycoside known in traditional Chinese medicine on vascular reactivity subsequent to high glucose stress. METHODS: The thoracic aortic rings with or without endothelium from male SD rats were mounted in an organ bath. Isometric contraction of aortic rings was measured. HO-1 protein expression and HO activity were also evaluated. RESULTS: (1) After incubation with 44 mmol/L of high glucose for 2 or 4 h, the vascular contraction responses to phenylephrine (PE) and relaxation response to acetylcholine (Ach) decreased in an endothelium-dependent manner; (2) Coincubation with puerarin (10(-10)-10(-8) mol/L) and high glucose, the high glucose-induced vasoconstriction and vasodilation dysfunction was partly inhibited in a dose-dependent manner; (3) Puerarin increased the HO-1 protein expression and HO activity of thoracic aorta. ZnPP (an inhibitor of heme oxygenase-1) offset the protective effect of puerarin. CONCLUSION: Puerarin could alleviate the high glucose-induced acute endothelium-dependent vascular dysfunction in rat aortic rings. HO-1 activity was proposed as a mechanism to account for the protection of vascular responses by puerarin. https://greenmedinfo.com/article/puerarin-protects-against-high-glucose-induced-acute-vascular-dysfunction#comments Endothelial Dysfunction Puerarin Antioxidants Heme oxygenase-1 up-regulation Antioxidants endothelial dysfunction Heme oxygenase-1 up-regulation Puerarin In Vitro Study Wed, 06 Jun 2018 23:20:26 +0000 greenmedinfo 165386 at https://greenmedinfo.com Puerarin protects against ischemic brain injury in a rat model of transient focal ischemia. https://greenmedinfo.com/article/puerarin-protects-against-ischemic-brain-injury-rat-model-transient-focal-isch n/a PMID:  Neurol Res. 2009 May ;31(4):402-6. PMID: 19508827 Abstract Title:  Puerarin protects against ischemic brain injury in a rat model of transient focal ischemia. Abstract:  OBJECTIVES: This study examines the efficacy of puerarin, a drug used in traditional Chinese medicine, in attenuating ischemic brain injury after cerebral ischemia and reperfusion, and explores possible mechanisms underlying neuroprotective effects. METHODS: The animal model of ischemia/reperfusion injury was induced by middle cerebral artery occlusion for 2 hours followed by up to 72 hour reperfusion. The rats were randomly assigned into four groups (n=6/group): puerarin at 100, 200 and 400 mg/kg or saline, administered intraperitoneally. Neurological outcome and infarct volume by 2% triphenyl tetrazolium chloride staining were determined 72 hours after reperfusion. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling staining was used to detect the cell damage of brains (n=5/group). Erythropoietin activation was detected by enzyme-linked immunosorbent assay (n=5/group). RESULTS: Compared with the vehicle saline group, puerarin decreased infarction volume at doses of 200 mg/kg (p=0.045) and 400 mg/kg (p=0.0002), but not at 100 mg/kg (p=0.387). Functional neurological outcome was improved with puerarin at 400 mg/kg (p=0.015), but not at 100 mg/kg (p=0.68) or 200 mg/kg (p=0.056). Puerarin significantly decreased the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling staining cells compared with the vehicle group 4, 24 and 72 hours after reperfusion. The erythropoietin activity was higher in puerarin treated group compared with the vehicle group. DISCUSSION: Puerarin has neuroprotection effects in rats at doses of 200 and 400 mg/kg, administered intraperitoneally after transient middle cerebral artery occlusion which may be partly due to activation of erythropoietin activity. https://greenmedinfo.com/article/puerarin-protects-against-ischemic-brain-injury-rat-model-transient-focal-isch#comments Middle Cerebral Artery Occlusion (MCAO) Puerarin Neuroprotective Agents Middle Cerebral Artery Occlusion (MCAO) Neuroprotective Agents Puerarin Animal Study Wed, 06 Jun 2018 23:30:02 +0000 greenmedinfo 165388 at https://greenmedinfo.com Puerarin protects PC12 cells against beta-amyloid-induced cell injury. https://greenmedinfo.com/article/puerarin-protects-pc12-cells-against-beta-amyloid-induced-cell-injury n/a PMID:  Cell Biol Int. 2008 Oct ;32(10):1230-7. Epub 2008 Jul 16. PMID: 18675923 Abstract Title:  Puerarin protects PC12 cells against beta-amyloid-induced cell injury. Abstract:  beta-Amyloid protein (Abeta), a major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. In this study, the protective effect of puerarin, an isoflavone purified from the radix of the Chinese herb Pueraria lobata, on Abeta-induced rat pheochromocytoma (PC12) cultures was investigated. Although exposure of PC12 cells to 50 microM Abeta25-35 caused significant viability loss and apoptotic rate increase, pretreatment of the cells with puerarin for 24h reduced the viability loss and apoptotic rate. Puerarin (1 microM) significantly inhibited Abeta25-35-induced apoptosis of PC12 cells. Preincubation of the cell with puerarin also restored the ROS and mitochondrial membrane potential levels that had been altered as a result of Abeta25-35 treatment. Puerarin was also found to increase the Bcl-2/Bax ratio and reduce caspase-3 activation. These results suggest that puerarin could attenuate Abeta25-35-induced PC12 cell injure and apoptosis and could also promote the survival of PC12 cells. Therefore, puerarin may act as an intracellular ROS scavenger, and its antioxidant properties may protect against Abeta25-35-induced cell injury. https://greenmedinfo.com/article/puerarin-protects-pc12-cells-against-beta-amyloid-induced-cell-injury#comments Brain: Oxidative Stress Puerarin Antioxidants Neuroprotective Agents Antioxidants Brain: Oxidative Stress Neuroprotective Agents Puerarin In Vitro Study Wed, 06 Jun 2018 23:05:20 +0000 greenmedinfo 165382 at https://greenmedinfo.com Puerarin seems to have certain protective effects on diabetic nephropathy. https://greenmedinfo.com/article/puerarin-seems-have-certain-protective-effects-diabetic-nephropathy n/a PMID:  Natl Med J India. 2009 Jan-Feb;22(1):9-12. PMID: 19761151 Abstract Title:  Effect of puerarin on the expression of extracellular matrix in rats with streptozotocin-induced diabetic nephropathy. Abstract:  BACKGROUND: Inhibition of the formation of advanced glycation end-products delays the development of diabetic nephropathy. Puerarin decreases the formation of these products. We studied the effect of puerarin in a rat model of diabetic nephropathy. METHODS: Three groups of rats were studied: a control group, a diabetic group in whom diabetic nephropathy was induced by intraperitoneal injection of streptozotocin, and a puerarin group in which diabetic rats were treated with puerarin. During and after treatment, measurements were made on the rats' general status, blood glucose level, blood urea nitrogen, serum creatinine, creatinine clearance rate and urinary albumin excretion over 24 hours. The expression of collagen I and heparan sulphate proteoglycan in the extracellular matrix of the glomerulus was assessed by immunohistochemistry. RESULTS: Rats in the puerarin group had a better general condition than those with diabetes. They also had lower blood urea nitrogen, serum creatinine and urinary albumin excretion rate over 24 hours compared with those in the diabetic group. The creatinine clearance and expression of heparan sulphate proteoglycan in the kidney also increased significantly in the puerarin group compared with that in the diabetic group. CONCLUSION: Puerarin seems to have certain protective effects on diabetic nephropathy induced by streptozotocin. This is caused possibly by regulating the expression of glomerular extracellular matrix. https://greenmedinfo.com/article/puerarin-seems-have-certain-protective-effects-diabetic-nephropathy#comments Diabetic Nephropathy Puerarin Renoprotective Diabetic Nephropathy Puerarin Renoprotective Animal Study Thu, 07 Jun 2018 00:16:24 +0000 greenmedinfo 165394 at https://greenmedinfo.com Puerarin shows a protective effect on the T2DM caused oxidative damage. https://greenmedinfo.com/article/puerarin-shows-protective-effect-t2dm-caused-oxidative-damage n/a PMID:  Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Aug ;29(8):707-10. PMID: 19848202 Abstract Title:  [Effect of puerarin on liver injury in KKAy mice with type 2 diabetes mellitus]. Abstract:  OBJECTIVE: To study the possible pathogenic mechanism of liver injury in type 2 diabetes mellitus (T2DM) and the intervening effect of puerarin on it. METHODS: Mice with T2DM (KKAy) were randomly divided into two groups, the model group and the puerarin group. And the C57BL/J mice of the same age were set up as normal controls. They were sacrificed at 28 weeks old for observing serum fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) by automatic biochemistry; liver cell apoptosis by flow cytometry; pathomorphology by electron microscope; and mRNA expressions of bcl-2 and bax genes by RT-PCR; as well as the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Na(+)-K(+)-ATPase; and content of malondialdehyde (MDA) in liver tissue by spectrophotometer. RESULTS: In KKAy mice, blood levels of FBG, TG, TC, ALT, AST and liver cell apoptosis rate were higher; the bax mRNA expression was higher and bcl-2 mRNA was lower markedly; the activities of SOD, GSH-Px, Na(+)-K(+)-ATPase in liver tissue were lower, and MDA content was higher than those in the normal control significantly (all P<0.01). Besides, mitochondria swelling and damage were found in liver tissue. While in the puerarin group after treatment, all the above-mentioned changes were alleviated to some extent. CONCLUSIONS: Obvious liver injury emerges in KKAy mice. Puerarin shows a protective effect on the T2DM caused oxidative damage by way of up-regulating bcl-2 to inhibit oxidative stress, and improving the energy metabolic dysfunction in liver of mice. https://greenmedinfo.com/article/puerarin-shows-protective-effect-t2dm-caused-oxidative-damage#comments Diabetes Mellitus: Type 2 Diabetes: Oxidative Stress Puerarin Antioxidants Antioxidants Diabetes mellitus: Type 2 Diabetes: Oxidative Stress Phytotherapy Puerarin Animal Study Thu, 07 Jun 2018 00:04:48 +0000 greenmedinfo 165391 at https://greenmedinfo.com This data contributes to a better understanding the mechanism of puerarin cardioprotection in the treatment of cardiovascular diseases. https://greenmedinfo.com/article/data-contributes-better-understanding-mechanism-puerarin-cardioprotection-trea n/a PMID:  Sci Rep. 2016 Oct 20 ;6:35475. Epub 2016 Oct 20. PMID: 27762288 Abstract Title:  The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism. Abstract:  Puerarin, a known isoflavone, is commonly found as a Chinese herb medicine. It is widely used in China to treat cardiac diseases such as angina, cardiac infarction and arrhythmia. However, its cardioprotective mechanism remains unclear. In this study, puerarin significantly prolonged ventricular action potential duration (APD) with a dosage dependent manner in the micromolar range on isolated rat ventricular myocytes. However, submicromolar puerarin had no effect on resting membrane potential (RMP), action potential amplitude (APA) and maximal velocity of depolarization (Vmax) of action potential. Only above the concentration of 10 mM, puerarin exhibited more aggressive effect on action potential, and shifted RMP to the positive direction. Millimolar concentrations of puerarin significantly inhibited inward rectified K(+) channels in a dosage dependent manner, and exhibited bigger effects upon Kir2.1 vs Kir2.3 in transfected HEK293 cells. As low as micromolar range concentrations of puerarin significantly inhibited Kv7.1 and IKs. These inhibitory effects may due to the direct inhibition of puerarin upon channels not via the PKA-dependent pathway. These results provided direct preclinical evidence that puerarin prolonged APD via its inhibitory effect upon Kv7.1 and IKs, contributing to a better understanding the mechanism of puerarin cardioprotection in the treatment of cardiovascular diseases. https://greenmedinfo.com/article/data-contributes-better-understanding-mechanism-puerarin-cardioprotection-trea#comments Cardiovascular Diseases Puerarin Cardioprotective Cardiovascular Diseases Chinese herb medicine Heart Disease Puerarin In Vitro Study Fri, 02 Dec 2016 21:30:25 +0000 greenmedinfo 139811 at https://greenmedinfo.com