5-Lipoxygenase Inhibitor https://greenmedinfo.com/category/keywords/5-Lipoxygenase%20Inhibitor en This report confirms the anti-inflammatory potential of this traditionally employed herbal medicine in vitro. https://greenmedinfo.com/article/report-confirms-anti-inflammatory-potential-traditionally-employed-herbal-medi n/a PMID:  Cell Immunol. 2012 ;272(2):283-9. Epub 2011 Oct 10. PMID: 22063737 Abstract Title:  Anti-inflammatory potential of an ethyl acetate fraction isolated from Justicia gendarussa roots through inhibition of iNOS and COX-2 expression via NF-κB pathway. Abstract:  Justicia gendarussa Burm.f. (J. gendarussa) is a plant used as traditional medicine in different parts of India and China to treat inflammatory disorders like rheumatoid arthritis. But its mechanism of anti-inflammatory action is still unclear. Hence in this context, the objective of our study is to reveal the mechanism of anti-inflammatory activity of J. gendarussa which would form an additional proof to the traditional knowledge of this plant. The anti-inflammatory function and mechanism(s) of action was studied in an ethyl acetate fraction isolated from methanolic extract of J. gendarussa roots (EJG). Anti-inflammatory studies were conducted on rats using partitioned fractions isolated from methanolic extract of J. gendarussa roots. In carrageenan-induced rat paw edema, ethyl acetate fraction brought about 80% and 93% edema inhibition at 3rd and 5th hour at a dose of 50 mg/kg, when compared to other extracts and Voveran. We investigated whether EJG inhibits the release of cycloxygenase (COX), 5-lipoxygenase (5-LOX), interleukin-6 (IL-6) and nuclear factor kappa B (NF-κB) in LPS stimulated human peripheral blood mononuclear cells (hPBMCs). Results shows that EJG dose dependently inhibited LPS-activated COX, 5-LOX, IL-6, and NF-κB in hPBMCs. EJG also reduced LPS induced levels of iNOS and COX-2 mRNA expression in hPBMCs. This study provides an insight into the probable mechanism(s) underlying the anti-inflammatory activity of EJG and therefore, we report the first confirmation of the anti-inflammatory potential of this traditionally employed herbal medicine in vitro. https://greenmedinfo.com/article/report-confirms-anti-inflammatory-potential-traditionally-employed-herbal-medi#comments Inflammation Justicia gendarussa Lipopolysaccharide-Induced Toxicity 5-lipoxygenase Inhibitor Anti-Inflammatory Agents Cyclooxygenase 2 Inhibitors Interleukin-6 Downregulation NF-kappaB Inhibitor 5-Lipoxygenase Inhibitor Anti-Inflammatory Agents Cyclooxygenase 2 Inhibitors Inflammation Interleukin-6 Downregulation Justicia gendarussa Lipopolysaccharide-Induced Toxicity NF-kappaB Inhibitor Plant Extracts In Vitro Study Thu, 15 Jun 2017 17:08:30 +0000 greenmedinfo 149182 at https://greenmedinfo.com