Horseradish https://greenmedinfo.com/category/keywords/Horseradish en Natural isothiocyanates represent a promising group of natural anti-infective compounds with activity against Pseudomonas aeruginosa biofilms. https://greenmedinfo.com/article/natural-isothiocyanates-represent-promising-group-natural-anti-infective-compo n/a PMID:  Fitoterapia. 2017 Jun ;119:57-63. Epub 2017 Apr 5. PMID: 28390975 Abstract Title:  Natural isothiocyanates express antimicrobial activity against developing and mature biofilms of Pseudomonas aeruginosa. Abstract:  BACKGROUND: The antimicrobial properties of natural isothiocyanates (ITCs) found in plants such as nasturtium (Tropaeolum majus) and horseradish (Armoracia rusticana), and the need of new chemotherapeutic options for treatment of infections caused by multidrug-resistant and biofilm-forming Gram-negative bacteria such as Pseudomonas aeruginosa (Pa), led us to evaluate the effects of three major ITCs, allylisothiocyanate (AITC), benzylisothiocyanate (BITC), and phenylethyl-isothiocyanate (PEITC), and a mixture (ITCM) adapted to the ITC composition after release of active components out of natural sources. MATERIAL/METHODS: Out of 105Pa isolates 27 isolates with increased biofilm formation were selected for testing. The effects of ITCs on Pa were evaluated regarding (1) planktonic bacterial proliferation, (2) biofilm formation, (3) metabolic activity in mature biofilms, and (4) synergism of ITCs and antibiotics. RESULTS: (1) Each ITC had anti-Pa activity. Mean minimum inhibitory concentrations (MICs) were (μg/ml, mean±standard deviation): AITC 103±6.9; BITC, 2145±249; PEITC 29,423±1652; and ITCM, 140±5. (2) Treating bacteria with PEITC and ITCM in concentrations below the MIC significantly inhibited biofilm formation. Particularly, ITCM reduced biofilm mass and bacterial proliferation. (3) ITCssignificantly inhibited metabolic activity in mature biofilms. (4) Combining ITCs with meropenem synergistically increased antimicrobial efficacy on Pa biofilms. CONCLUSIONS: ITCs represent a promising group of natural anti-infective compounds with activity against Pa biofilms. https://greenmedinfo.com/article/natural-isothiocyanates-represent-promising-group-natural-anti-infective-compo#comments Horseradish Isothiocyanates Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Bacterial Agents Biofilm Horseradish Isothiocyanates Pseudomonas aeruginosa In Vitro Study Tue, 02 Jan 2018 19:41:26 +0000 greenmedinfo 157921 at https://greenmedinfo.com The results suggest that horseradish root exerts an antiphlogistic activity in human immune cells. https://greenmedinfo.com/article/results-suggest-horseradish-root-exerts-antiphlogistic-activity-human-immune-c n/a PMID:  Evid Based Complement Alternat Med. 2017 ;2017:1950692. Epub 2017 Jan 15. PMID: 28182113 Abstract Title:  Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction. Abstract:  Horseradish (Armoracia rusticana) is a perennial crop and its root is used in condiments. Traditionally, horseradish root is used to treat bacterial infections of the respiratory tract and urinary bladder. The antiphlogistic activity, determined in activated primary human peripheral blood mononuclear cells (PBMC), was evaluated for an aqueous extract and its subfractions, separated by HPLC. Compound analysis was done by UHPLC-QToF/MS and GC-MS. The aqueous extract concentration-dependently inhibited the anti-inflammatory response to lipopolysaccharide (LPS) in terms of TNF-α release at ≥37 μg/mL. Further, the cyclooxygenase as well as lipoxygenase pathway was blocked by the extract as demonstrated by inhibition of COX-2 protein expression and PGE2 synthesis at ≥4 μg/mL and leukotriene LTB4 release. Mechanistic studies revealed that inhibition of ERK1/2 andc-Jun activation preceded COX-2 suppression upon plant extract treatment in the presence of LPS. Chemical analysis identified target compounds with a medium polarity as relevant for the observed bioactivity. Importantly, allyl isothiocyanate, which is quite well known for its anti-inflammatory capacity and as the principal pungent constituent in horseradish roots, was not relevant for the observations. The results suggest that horseradish root exerts an antiphlogistic activity in human immune cells by regulation of the COX and LOX pathway via MAPK signalling. https://greenmedinfo.com/article/results-suggest-horseradish-root-exerts-antiphlogistic-activity-human-immune-c#comments Horseradish Inflammation Lipopolysaccharide-Induced Toxicity Anti-Inflammatory Agents Cyclooxygenase 2 Inhibitors Prostaglandin PGE2 downregulation Anti-Inflammatory Agents Horseradish Inflammation Lipopolysaccharide-Induced Toxicity In Vitro Study Tue, 02 Jan 2018 20:07:28 +0000 greenmedinfo 157927 at https://greenmedinfo.com