Pleurotus nebrodensis https://greenmedinfo.com/category/keywords/Pleurotus%20nebrodensis en A polysaccharide isolated from Pleurotus nebrodensis shows immune-stimulating activity. https://greenmedinfo.com/article/polysaccharide-isolated-pleurotus-nebrodensis-shows-immune-stimulating-activit n/a PMID:  Chin J Nat Med. 2015 May ;13(5):355-60. PMID: 25986284 Abstract Title:  The polysaccharide isolated from Pleurotus nebrodensis (PN-S) shows immune-stimulating activity in RAW264.7 macrophages. Abstract:  A novel Pleurotus nebrodensis polysaccharide (PN-S) was purified and characterized, and its immune-stimulating activity was evaluated in RAW264.7 macrophages. PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner, as determined by the MTT assay. After exposure to PN-S, the phagocytosis of the macrophages was significantly improved, with remarkable changes in morphology being observed. Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases. PN-S treatment enhanced the productions of interleukin-6 (IL-6), nitric oxide (NO), interferon gamma (INF-γ), and tumor necrosis factor-α (TNF-α) in the macrophages, with up-regulation of mRNA expressions of interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), interferon gamma(INF-γ) and tumor necrosis factor-α (TNF-α) being observed in a dose-dependent manner, as measured by qRT-PCR. Inconclusion, these results suggest that the purified PN-S can improve immunity by activating macrophages. https://greenmedinfo.com/article/polysaccharide-isolated-pleurotus-nebrodensis-shows-immune-stimulating-activit#comments Pleurotus nebrodensis Immunostimulatory Immunostimulatory Pleurotus nebrodensis In Vitro Study Sat, 10 Feb 2018 07:34:23 +0000 greenmedinfo 159679 at https://greenmedinfo.com Polysaccharide from Pleurotus nebrodensis induces apoptosis via a mitochondrial pathway in HepG2 cells. https://greenmedinfo.com/article/polysaccharide-pleurotus-nebrodensis-induces-apoptosis-mitochondrial-pathway-h n/a PMID:  Food Funct. 2016 Jan ;7(1):455-63. PMID: 26506946 Abstract Title:  Polysaccharide from Pleurotus nebrodensis induces apoptosis via a mitochondrial pathway in HepG2 cells. Abstract:  A novel alkali extractable polysaccharide (designated as PNA-2) was purified from Pleurotus nebrodensis and the effects of purified PNA-2 on the proliferation and apoptosis of human hepatic cancer cells (HepG2) were investigated in this study. The results of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that PNA-2 inhibited the proliferation of HepG2 cells by apoptosis induction, which was also characterized using scanning electron microscopy (SEM). Moreover, the expression of apoptosis-associated mRNA, proteins and the cell-cycle arrest at the G0/G1 phase was determined using RT-qPCR, Western blot and flow cytometry, respectively. A notable inhibition of the migration rate of PNA-2-treated HepG2 cells was observed using a cell scratch assay. DNA damage was observed using a comet assay and AO/EB staining in HepG2 cells, which were exposed to PNA-2. Induction of the mitochondria-mediated intrinsic apoptotic pathway by PNA-2 was indicated by the loss of mitochondrial membrane potential (ΔΨm), Bcl-2 dysregulation and cytochrome c release. All the results suggested that the mitochondria-mediated intrinsic apoptotic pathway could be involved in PNA-2-mediated apoptosis of human liver carcinoma cells HepG2. Finally, the results indicated that PNA-2 significantly suppressed tumor growth in HepG2 tumor-bearing mice, indicating that PNA-2 may be developed as a candidate drug or functional food factor to prevent or treat liver cancer. https://greenmedinfo.com/article/polysaccharide-pleurotus-nebrodensis-induces-apoptosis-mitochondrial-pathway-h#comments DNA damage Pleurotus nebrodensis Antineoplastic Agents Apoptotic Antineoplastic Agents Apoptotic DNA damage Pleurotus nebrodensis Polysaccharides Animal Study In Vitro Study Sat, 10 Feb 2018 07:27:37 +0000 greenmedinfo 159677 at https://greenmedinfo.com