Matrix metalloproteinase-9 (MMP-9) inhibitor https://greenmedinfo.com/category/keywords/Matrix%20metalloproteinase-9%20%28MMP-9%29%20inhibitor en Hesperidin inhibited the migratory and invasive capabilities of A549 human non-small cell lung cancer cells. https://greenmedinfo.com/article/hesperidin-inhibited-migratory-and-invasive-capabilities-a549-human-non-small- n/a PMID:  Life Sci. 2018 Mar 28. Epub 2018 Mar 28. PMID: 29604270 Abstract Title:  Hesperidin suppresses the migration and invasion of non-small cell lung cancer cells by inhibiting the SDF-1/CXCR-4 pathway. Abstract:  OBJECTIVE: The present study aimed to investigate the ability of hesperidin to suppress the migration and invasion of A549 cells, and to investigate the role of the SDF-1/CXCR-4 cascade in this suppression. METHODS: We performed a Transwell migration assay to measure the migratory capability of A549 cells treated with 0.5% DMSO, SDF-1α, AMD3100 or hesperidin. The SDF-1 level in the culture medium was determined by an enzyme-linked immunosorbent assay (ELISA) to detect whether different concentrations of hesperidin affected SDF-1 secretion. A wound-healing assay was performed to determine the effects of different concentrationsof hesperidin on the migration inhibition of A549, H460 and H1975 cells. Additionally, the effect of various hesperidin concentrations on the rate of A549 cell invasion and migration was examined with and without Matrigel in Transwell assays, respectively. Western blot analysis was used to evaluatethe protein levels of CXCR-4, MMP-9, CK-19, Vimentin, p65, p-p65, p-IκB, IκB, p-Akt and Akt. RT-qPCR was used to detect the mRNA levels of CXCR-4, MMP-9, CK-19, Vimentin, p65, IκB, SDF-1 and Akt. RESULTS: The Transwell migration assay indicated that SDF-1α promoted A549 cell migration, while AMD3100 and hesperidin significantly inhibited the migratory capability. The wound-healing assay demonstrated that hesperidin treatment significantly reduced the rate of wound closure compared with the control group in a dose-dependent manner. Similarly, the migration and invasive abilities of A549 cells, H460 and H1975 cells treated with hesperidin were significantly decreased compared with the control group. The ELISA data suggested that hesperidin attenuated the secretion of SDF-1 from A549 cells in a dose-dependent manner. Furthermore, western blot analysis indicated that SDF-1α treatment significantly increased the levels of CXCR-4, p-p65, p-IκB and p-Akt in A549 cells. In contrast, AMD3100 or hesperidin reversed the effect induced by SDF-1α through decreasing the expression of CXCR-4. Subsequent RT-qPCR and western blot analyses also confirmed that hesperidin had a significant effect on the expression of EMT-related proteins, including MMP-9, CK-19 and Vimentin, in A549 cells. CONCLUSION: In summary, we demonstrated that hesperidin inhibited the migratory and invasive capabilities of A549 human non-small cell lung cancer cells by the mediation of the SDF-1/CXCR-4 signaling cascade, thus providing the foundation for the development of hesperidin as a safer and more effective anticancer drug for non-small cell lung cancer. https://greenmedinfo.com/article/hesperidin-inhibited-migratory-and-invasive-capabilities-a549-human-non-small-#comments Hesperidin Non-Small-Cell Lung Carcinoma Anti-metastatic Matrix metalloproteinase-9 (MMP-9) inhibitor Anti-metastatic Hesperidin Matrix metalloproteinase-9 (MMP-9) inhibitor Non-Small-Cell Lung Carcinoma In Vitro Study Sat, 07 Apr 2018 02:45:56 +0000 greenmedinfo 162319 at https://greenmedinfo.com These findings suggested that betulinic acid might be a potential agent for inhibiting the growth and metastasis of breast cancer. https://greenmedinfo.com/article/these-findings-suggested-betulinic-acid-might-be-potential-agent-inhibiting-gr n/a PMID:  Oncotarget. 2018 Jan 9 ;9(3):3794-3804. Epub 2017 Dec 17. PMID: 29423083 Abstract Title:  Betulinic acid impairs metastasis and reduces immunosuppressive cells in breast cancer models. Abstract:  Breast cancer is the most common female cancer with considerable metastatic potential, explaining the need for new candidates that inhibit tumor metastasis. In our study, betulinic acid (BA), a kind of pentacyclic triterpenoid compound derived from birch trees, was evaluated for its anti-metastasis activityand. BA decreased the viability of three breast cancer cell lines and markedly impaired cell migration and invasion. In addition, BA could inhibit the activation of stat3 and FAK which resulted in a reduction of matrix metalloproteinases (MMPs), and increase of the MMPs inhibitor (TIMP-2) expression. Moreover, in our animal experiment, intraperitoneal administration of 10 mg/kg/day BA suppressed 4T1 tumor growth and blocked formation of pulmonary metastases without obvious side effects. Furthermore, histological and immunohistochemical analyses showed a decrease in MMP-9 positive cells, MMP-2 positive cells and Ki-67 positive cells and an increase in cleaved caspase-3 positive cells upon BA administration. Notably, BA reduced the number of myeloid-derived suppressor cells (MDSCs) in the lungs and tumors. Interestingly, in our caudal vein model, BA also obviously suppressed 4T1 tumor pulmonary metastases. These findings suggested that BA might be a potential agent for inhibiting the growth and metastasis of breast cancer. https://greenmedinfo.com/article/these-findings-suggested-betulinic-acid-might-be-potential-agent-inhibiting-gr#comments Betulinic acid Breast Cancer Anti-metastatic Matrix metalloproteinase-9 (MMP-9) inhibitor Anti-metastatic Betulinic acid Breast Cancer Matrix metalloproteinase-9 (MMP-9) inhibitor In Vitro Study Mon, 19 Feb 2018 15:11:28 +0000 greenmedinfo 160144 at https://greenmedinfo.com